Development of a quantitative immuno-affinity test column assay for on-site screening of clindamycin residues in milk |
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| Affiliation: | 1. Gubra, Hørsholm, Denmark;2. Department of Human Nutrition, University of Copenhagen, Frederiksberg, Denmark;3. Department of Biomedical Sciences, School of Veterinary Medicine, Cornell University, Ithaca, NY, USA;4. Department of Molecular Biosciences, University of California, Davis, CA, USA;1. Laboratory of Pharmacology, Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan;2. Department of Physiology, School of Allied Health Sciences, Kitasato University, Sagamihara 252-0373, Japan |
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| Abstract: | A sensitive and specific monoclonal antibody against clindamycin (CLIN) was produced and used to develop an immuno-affinity test column (IATC) assay for on-site screening of CLIN residues in milk. The qualitative limit of detection of the IATC assay, estimated as 1.0 μg L−1 by visual detection, was sufficient to measure maximum residue levels for lincosamide antibiotics in milk. The quantitative IATC assay resulted in a lower detection limit (0.11 μg L−1) by evaluating the colour intensity of the test layers, which was approximately 9 times greater sensitivity than visual detection. During the spike and recovery test, the average recoveries ranged from 76% to 114% at different spiked levels, and the intra-/interday coefficients of variation were in the range 9.6–16.7%. The detection time was shortened to 20 min, whereas the sensitivity was comparable with a traditional ELISA. The IATC assay shows promise for on-site screening of CLIN residues in milk. |
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