A novel staggered hybrid SSF approach for efficient conversion of cellulose/hemicellulosic fractions of corncob into ethanol |
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| Affiliation: | 1. Department of Microbiology, Guru Nanak Dev University, Amritsar, Punjab 143005, India;2. Department of Food Science & Technology, Guru Nanak Dev University, Amritsar, Punjab 143005, India;1. Engine Research Laboratory, Department of Mechanical Engineering, Indian Institute of Technology Kanpur, Kanpur 208016, India;2. Combustion Engine and Energy Conversion Laboratory, School of Mechanical Engineering, College of Engineering, Hanyang University, Seoul 133-791, Republic of Korea;1. Thermo-catalytic Processes Area (TPA), Bio-Fuels Division (BFD), CSIR-Indian Institute of Petroleum (IIP), Dehradun 248005, India;2. Academy of Scientific and Innovative Research (AcSIR), New Delhi, India;1. Engine Research Laboratory, Department of Mechanical Engineering, Indian Institute of Technology Kanpur, Kanpur 208016, India;2. Department of Civil Engineering, Indian Institute of Technology Kanpur, Kanpur 208016, India;1. Centre for Ocean Research, Sathyabama University, Jeppiaar Nagar, Rajiv Gandhi Road, Chennai 600119, Tamil Nadu, India;2. ESSO-National Institute of Ocean Technology (ESSO-NIOT), Pallikaranai, Chennai 600100, Tamil Nadu, India |
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| Abstract: | The following study reports bioconversion of corncob into ethanol using hybrid approach for co-utilization of dilute acid hydrolysate (pentose rich stream) and hexose rich stream obtained by enzymatic saccharification employing commercial cellulase Cellic CTec2 as well as in-house cellulase preparations derived from Malbranchea cinnamomea, Scytalidium thermophilium and a recombinant Aspergillus strain. Acid hydrolysis (1% H2SO4) of corncob at 1:15 solid liquid ratio led to removal of 80.5% of hemicellulosic fraction. The solid glucan rich fraction (63.5% glucan, 8.3% pentosans and 27.9% lignin) was hydrolysed at 10% substrate loading rate with different enzymes for 72 h at 50 °C resulting in release of 732 and 535 (mg/g substrate) total sugars by Cellic CTec2 and M. cinnamomea derived enzymes, respectively. The fermentation of enzyme hydrolysate with co-culture of Saccharomyces cerevisiae and Pichia stipitis added in sequential manner resulted in 3.42 and 2.50% (v/v) ethanol in hydrolysate obtained from commercial Cellic CTec2 and M. cinnamomea, respectively. Employing a hybrid approach, where dilute acid hydrolysate stream was added to solid residue along with enzyme Cellic CTec2 during staggered simultaneous saccharification and fermentation at substrate loading rate of 15% resulted in 252 g ethanol/kg corncob. |
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| Keywords: | Corncob residue Enzymatic hydrolysis Saccharification Cellic CTec2 |
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