Bioavailability of peptides from casein hydrolysate in vitro: Amino acid compositions of peptides affect the antioxidant efficacy and resistance to intestinal peptidases |
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| Affiliation: | 1. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China;2. Key Laboratory of Functional Dairy, Ministry of Education, Beijing 100083, China;1. Department of Bioscience, Federal University of São Paulo (UNIFESP), Silva Jardim Street 136, ZIP CODE: 11015-020 Santos, SP, Brazil;2. Department of Food Science and Experimental Nutrition, FCF, University of São Paulo, São Paulo, SP, Brazil;3. NAPAN — Food and Nutrition Research Center, São Paulo, Brazil;4. Food Research Center (FoRC), CEPID-FAPESP (Research, Innovation and Dissemination Centers, São Paulo Research Foundation), São Paulo, Brazil;5. Department of Food Science, Faculty of Food Engineering, University of Campinas (UNICAMP), Campinas, Brazil;1. Charles Viollette Institute EA7394/USC1281, Université Lille 1, Boulevard Paul Langevin, 59653 Villeneuve d''Ascq Cedex, France;2. CUMA, Faculté de Pharmacie, Lille, France;3. Univ Lille Nord de France, Unité Environnement Périnatal et Croissance, EA 4489, Equipe dénutritions maternelles périnatales, Université Lille 1, Bâtiment SN4, 59655 Villeneuve d''Ascq, France;1. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China;2. Beijing Higher Institution Engineering Research Center of Animal Product, Beijing 100083, China |
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| Abstract: | Measurements of in vitro bioactivity to support health benefits of bioactive compounds should be accomplished with estimates of their bioavailability to bolster nutritional significance to health claims. In vitro bioavailability of casein and casein peptides (casein hydrolysate and four peptide fractions) measured by the amount of peptide nitrogen is discussed. Antioxidant activities during gastrointestinal digestion and Caco-2 cell absorption were investigated as indices of peptide degradation. The antioxidant capacities of Trolox equivalent and oxygen radicals were used for assessing antioxidant efficacy of surviving peptides. Results showed that casein hydrolysate improved bioavailability compared to casein. Amino acid composition of peptides affected the resistance of peptides to digestive enzymes and intestinal peptidases. The acidic peptide fractions had higher bioavailability and a higher residual ratio of antioxidant activity. The peptides in the digest and absorbate of acidic fraction F1 with the highest bioavailability (23.14%) and the residual ratio of antioxidant activity were identified, and 12 intact, absorbed peptides (IAP) were obtained. Eleven of twelve of the IAPs were from β-Casein, and their amino acid components were rich in acidic and hydrophobic amino acids. Identification of IAPs might provide insight into the mechanism of how peptide structure provides resistance against peptidases by Caco-2 cells. |
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