Optimization of concomitant production of cellulase and xylanase from Rhizopus oryzae SN5 through EVOP-factorial design technique and application in Sorghum Stover based bioethanol production |
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| Affiliation: | 1. Department of Biotechnology, Motilal Nehru National Institute of Technology, Allahabad 211004, Uttar Pradesh, India;2. Bioenergy and Energy Planning Research Group, Swiss Federal Institute of Technology (EPFL), Lausanne, Switzerland;1. Engine Research Laboratory, Department of Mechanical Engineering, Indian Institute of Technology Kanpur, Kanpur 208016, India;2. Combustion Engine and Energy Conversion Laboratory, School of Mechanical Engineering, College of Engineering, Hanyang University, Seoul 133-791, Republic of Korea;1. Thermo-catalytic Processes Area (TPA), Bio-Fuels Division (BFD), CSIR-Indian Institute of Petroleum (IIP), Dehradun 248005, India;2. Academy of Scientific and Innovative Research (AcSIR), New Delhi, India;1. Centre for Ocean Research, Sathyabama University, Jeppiaar Nagar, Rajiv Gandhi Road, Chennai 600119, Tamil Nadu, India;2. ESSO-National Institute of Ocean Technology (ESSO-NIOT), Pallikaranai, Chennai 600100, Tamil Nadu, India;1. Engine Research Laboratory, Department of Mechanical Engineering, Indian Institute of Technology Kanpur, Kanpur 208016, India;2. Department of Civil Engineering, Indian Institute of Technology Kanpur, Kanpur 208016, India |
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| Abstract: | Current study deals with the production of cellulases and xylanases from the Rhizopus oryzae SN5 isolated from composed soil of Himalayan pine forest, in order to meet the challenges of lignocellulosic biomass based biorefineries. Culture parameters for concomitant production of cellulase and xylanase were optimized through EVOP-factorial design technique under solid state fermentation. And maximum yield of cellulase and xylanase were obtained 437.54 U/gds and 273.83 U/gds, respectively at 30 °C and pH 6.0 after 5 days of incubation. On applying these enzymes for the saccharification of the dilute acid pretreated Sorghum Stover (SS), 0.407 g/g sugar was yielded. This hydrolysate on fermentation, yielded 0.411 g/g ehanol with Saccharomyces cerevisiae (NCIM 3288), which could be considered a good conversion. Therefore, Rhizopus oryzae SN5 was found as potent strain for the production of the cocktail of lignocellulosic biomasss hydrolytic enzymes and would be promising tool in the area of lignocellulose based bio-refineries. |
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| Keywords: | Cellulase Xylanase Saccharification Ethanol SSF |
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