Molecularly imprinted polymer-high performance liquid chromatography for the determination of tetracycline drugs in animal derived foods |
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| Affiliation: | 1. Department of Analytical Chemistry, Nutrition and Bromatology, Faculty of Veterinary Medicine, University of Santiago de Compostela, 27002 Lugo, Spain;2. Laboratorio de Sanidade e Produccion Animal de Galicia, Avenida de Madrid 77, 27002 Lugo, Spain;1. Key Laboratory of Watershed Sciences and Health of Zhejiang Province, Wenzhou Medical University, Wenzhou 325035, China;2. College of Life Sciences, Wenzhou Medical University, Wenzhou 325035, China;1. Department of Chemistry, Saveh Branch, Islamic Azad University, Saveh, Iran;2. Department of Materials Science and Engineering, K.N. Toosi University of Technology, Tehran, Iran;1. Department of Chemistry, Saveh Branch, Islamic Azad University, Saveh, Iran;2. Department of Materials Science and Engineering, K.N. Toosi University of Technology, Tehran, Iran |
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| Abstract: | In this study, a molecularly imprinted polymer solid phase extraction method combining high performance liquid chromatography was developed to determine the residues of tetracycline drugs in animal derived foods. The polymer was synthesized with chlortetracycline as template molecule, methacrylic acid as functional monomer at template/monomer ratio of 1:4. The prepared solid phase extraction column was able to capture four tetracyclines simultaneously with high adsorption capacities (3560–4700 ng) and high recoveries (>87%), and was able to be reused for at least thirty times. The limits of detection were in a range of 20–40 ng/g, and the recoveries from fortified blank samples (milk, egg and pork) were in a range of 74%–93%. Furthermore, the polymer-based column achieved better purification effect than three commercial solid phase extraction columns. This is the first study reporting the use of chlortetracycline based molecularly imprinted polymer for determination of tetracyclines in animal derived foods. |
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| Keywords: | Molecularly imprinted polymer Solid phased extraction High performance liquid chromatography Tetracycline drugs |
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