河蚬多糖分离纯化及抗氧化、抗肿瘤活性研究

采用DEAE-52纤维素色谱法和Sephadex~(TM) G-100葡聚糖凝胶色谱法对河蚬多糖进行分离纯化,获得CFP-1和CFP-2两个纯化组分,并对两组分进行紫外光谱和红外光谱扫描,结果表明:两组分中没有蛋白质和核酸,并证明它们是多糖类物质。液相色谱分析得到CFP-1和CFP-2的平均相对分子质量分别为1 172,3 627kD。对河蚬多糖的抗肿瘤、抗氧化活性研究结果表明:CFP-1对人肝癌细胞(HepG-2)抑制作用较CFP-2明显,作用48h时,CFP-1对人肝癌细胞的抑制活性IC_(50)值为0.24 mg/mL;而在抗氧化活性方面CFP-2优于CFP-1。

Study onisolation, antioxidation and anti-tumor activites of polysaccharide in Corbicula fluminea

CFP-1 and CFP-2 were purified from Corbicula fluminea by DEAE-52 cellulose anion-exchange chromatography and Sephadex^TMG-100 gel-filfration chromatography. The molecule structure was analyzed by ultraviolet spectrum scanning and infraned spectrum. Ultraviolet spectrum showed that CFP-1 and CFP-2 contained little DNA and protein, and infraned spectrum showed that both of them were polysaccharides. Purity of CFP-1 and CFP-2 was further confirmed by using HPLC, they were homogenous with a molecular weight of 1 172 kD and 3 627 kD. The result showed that CFP-1 could significantly inhibit proliferation of HepG-2 than CFP-2. with the IC₅₀ value of 0.24 mg/mL after 48 h treating. In addition, radical scavenging tests revealed that CFP-2 showed significant function of antioxidation activity.