梓树根皮总黄酮分离纯化及其抑菌活性研究

以吸附率和解析率为评价指标,用大孔树脂对梓树根皮总黄酮的分离纯化工艺进行优化,并研究纯化后梓树根皮总黄酮的抑菌活性。结果表明,梓树根皮总黄酮分离纯化最佳工艺条件为:采用NKA-II树脂,上样液质量浓度0.538mg/mL,上样液流速1.66 mL/min,上样液pH值4.01,洗脱剂乙醇浓度70%,洗脱流速2.5 mL/min。该条件下,梓树根皮总黄酮纯度为(77.43±0.23)%,吸附率为(93.36±0.25)%,解析率为(95.51±0.17)%。抑菌活性表明,梓树根皮总黄酮对大肠杆菌、枯草芽孢杆菌、金黄色葡萄球菌抑菌作用为极敏,对青霉菌、酿酒酵母菌抑菌作用为高敏;金黄色葡萄球菌的MIC值为(6.25±0.25)mg/mL;枯草芽孢杆菌、大肠杆菌MIC值为(12.50±0.36)mg/mL;酿酒酵母、青霉菌,MIC值为(25.00±0.27)mg/mL。

Separation,purification and antibacterial activity of total flavonoids from Catalpa root bark

Taking the adsorption rate and desorption rate as the evaluation indexes, macroporous resin was adopted to optimize the separation and purification technology of total flavonoids from Catalpa root bark, and the antibacterial activity of the purified total flavonoids from Catalpa root bark was investigated. The results indicated that the optimal technological conditions for the separation and purification of total flavonoids from Catalpa root bark were as follows: application of NKA-II resin; mass concentration, flow rate and pH value of loading buffer of 0.538 mg/mL, 1.66 mL/min and 4.01, respectively; concentration of eluant ethyl alcohol of 70%; and elution flow rate of 2.5 mL/min. Under such conditions, the purity, adsorption rate and desorption rate of total flavonoids from Catalpa root bark were (77.43±0.23)%, (93.36±0.25)% and (95.51±0.17)%, respectively. Antibacterial activity indicated that total flavonoids from Catalpa root bark had extremely sensitive antibacterial activity to Escherichia coli, Bacillus subtilis, and Staphylococcus aureus; and high antibacterial sensitivity to penicillium and Saccharomyces cerevisiae. MIC values of S. aureus, Bacillus subtilis and E. coli, as well as S. cerevisiae and penicillium were (6.25±0.25) mg/mL, (12.50±0.36) mg/mL and (25.00±0.27) mg/mL, respectively.