| 琥珀酸产生菌的诱变与选育 |
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| 引用本文： | 林剑,孙莹,刘晓艳,徐世艾.琥珀酸产生菌的诱变与选育[J].食品科学,2007,28(12):309-312. |
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| 作者姓名： | 林剑 孙莹 刘晓艳 徐世艾 |
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| 作者单位： | 烟台大学化工制造省级重点实验室； 烟台大学化工制造省级重点实验室 山东烟台264005； 山东烟台264005； |
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| 摘 要： | 对琥珀酸产生菌S-1进行紫外线亚硝基胍的复合诱变后,筛选出琥珀酸产量高,遗传性状稳定的菌株S-57,并对其进行激光诱变,筛选出菌株SH-24,相同发酵条件下琥珀酸产量达到21.25g/L,相对于未诱变菌株产量提高了343倍。
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| 关 键 词： | 琥珀酸 紫外线 亚硝基胍 激光 诱变 |
| 文章编号： | 1002-6630（2007）12-0309-04 |
| 收稿时间： | 2006-10-31 |
| 修稿时间： | 2006年10月31 |
Mutagenesis and Screening of Succinic-producing Strain |
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| LIN Jian,SUN Ying,LIU Xiao-yan,XU Shi-ai.Mutagenesis and Screening of Succinic-producing Strain[J].Food Science,2007,28(12):309-312. |
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| Authors: | LIN Jian SUN Ying LIU Xiao-yan XU Shi-ai |
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| Affiliation: | Provincial Key Laboratory of Chemical Industry Manufacture, Yantai University, Yantai 264005, China |
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| Abstract: | A high yield and stable succinic producing strain S-57 was obtained from the mutation of mucor S-1 treated with ultraviolet ray and nitrosoguanidine (NTG). After the strain S-57 was treated with He-Ne laser, a strain SH-24 was selected. The concentration of succinic acid is 21.25 mg/ml in the fermentation broth of the same conditions of fermentation or that is 343 times the strain before mutagenesis. |
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| Keywords: | succinic acid sltraviolet ray nitrosoguanidine laser mutation |
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