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| 亚氯酸钠对鲜烟酶促棕色化反应的影响 | |
| 引用本文： | 任杰,张勤玉,赵晓超,孙福山,任百战,程森,赵鹏,高远,肖头杰,徐世峰. 亚氯酸钠对鲜烟酶促棕色化反应的影响[J]. 中国烟草科学, 2012, 33(5): 63-67. DOI: 10.3969/j.issn.1007-5119.2012.05.012 |
| 作者姓名： | 任杰 张勤玉 赵晓超 孙福山 任百战 程森 赵鹏 高远 肖头杰 徐世峰 |
| 作者单位： | 1. 中国农业科学院烟草研究所, 农业部烟草生物学与加工重点实验室, 青岛 266101; 2. 陕西富安生物科技有限公司, 陕西 宝鸡 721013; 3. 湖北中烟工业有限责任公司, 武汉 430040; 4. 陕西省烟草公司延安市公司, 陕西 延安 716000; 5. 上海烟草集团有限责任公司, 上海 200082; 6. 陕西省烟草公司安康市公司, 陕西 安康 725000 |
| 基金项目： | 中国农业科学院烟草研究所所长基金项目(TR2011-10);上海烟草集团有限责任公司项目(2011-00600) |
| 摘 要： | 以烤烟K326为试验材料, 通过暗箱试验研究了不同浓度亚氯酸钠(SC), 叶面喷施和浸泡两种处理方式对鲜烟酶促棕色化反应的影响。结果表明, 200 mg/L SC对鲜烟酶促棕色化反应的抑制作用最为显著。喷施和浸泡两种处理方式对酶促棕色化反应的影响无显著差异。SC对不同成熟度鲜烟酶促棕色化反应抑制效果不同, 其中以M3成熟度(9~10成黄绿)效果最好。SC处理和对照烟叶多酚氧化酶(PPO)活性变化表现出基本相同的规律, 但SC处理烟叶PPO活性均低于同期对照烟叶。 |
| 关 键 词： | 亚氯酸钠 酶促棕色化反应 烤烟 PPO |
| 收稿时间： | 2011-11-13 |
Effects of Sodium Chlorite on Enzymatic Browning Reaction of Flue-cured Tobacco Leaves |
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| REN Jie,ZHANG Qinyu,ZHAO Xiaochao,SUN Fushan,REN Baizhan,CHENG Sen,ZHAO Peng,GAO Yuan,XIAO Toujie,XU Shifeng. Effects of Sodium Chlorite on Enzymatic Browning Reaction of Flue-cured Tobacco Leaves[J]. Chinese Tobacco Science, 2012, 33(5): 63-67. DOI: 10.3969/j.issn.1007-5119.2012.05.012 | |
| Authors: | REN Jie ZHANG Qinyu ZHAO Xiaochao SUN Fushan REN Baizhan CHENG Sen ZHAO Peng GAO Yuan XIAO Toujie XU Shifeng |
| Affiliation: | 1.Tobacco Research Institute of CAAS,Key Laboratory of Tobacco Biology and Processing,Ministry of Agriculture,Qingdao 266101;2.Shaanxi Fuan Biological Science and Technology Co.,Ltd.,Baoji Shaanxi 721013,China;3.China Tobacco Hubei Industrial Co.,Ltd.,Wuhan 430040,China;4.Yan’an Branch of Shaanxi Tobacco Company,Yan’an,Shaanxi 716000,China;5.Shanghai Tobacco Group Co.,Ltd.,Shanghai 200082,China;6.Ankang Branch of Shaanxi Tobacco Company,Ankang,Shaanxi 725000,China) |
| Abstract: | Effects of sodium chlorite (SC) of different concentrations and treatment methods of leaf surface spaying and dipping leaves on enzyme browning reaction of K326 flue-cured tobacco were investigated. The results indicated that 200 mg/L SC application markedly inhibit enzyme browning reaction of tobacco leaves. There were no significantly different efficacy in reducing browning of two treatment methods of leaf surface spaying and dipping leaves. There were different effects of SC on enzyme browning reaction of tobacco leaves of different maturity. SC application on M3 maturity leaves was found to achieve even better inhibition of browning than other maturity leaves. Changes of PPO activity of tobacco leaves treated with SC and water respectively were similar, but PPO activity of tobacco leaves treated with SC was lower than control tobacco leaves. |
| Keywords: | sodium chlorite enzyme browning reaction flue-cured tobacco PPO |
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