| Nano-HA涂层与HA/CTS复合涂层抑制胶质母细胞瘤细胞活力作用的比较 |
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| 引用本文： | 田昂,王超,管格非,薛向欣,吴安华,王丽.Nano-HA涂层与HA/CTS复合涂层抑制胶质母细胞瘤细胞活力作用的比较[J].功能材料,2010,41(2). |
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| 作者姓名： | 田昂 王超 管格非 薛向欣 吴安华 王丽 |
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| 作者单位： | 1. 东北大学,材料与冶金学院,辽宁,沈阳,110004
2. 中国医科大学,第一附属医院,神经外科,辽宁,沈阳,110001 |
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| 基金项目： | 国家自然科学基金资助项目(50872019) |
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| 摘 要： | 实验采用电化学沉积法在钛合金表面制备了纳米羟基磷灰石涂层(nHA)、纳米和微米级羟基磷灰石/壳聚糖复合涂层(nHA/CTS,mHA/CTS),并应用XRD、SEM和FTIR对涂层的理化特性进行了表征。然后将人脑胶质母细胞瘤细胞系U87(U87)与3种涂层共培养,并比较3种涂层诱导U87细胞凋亡的能力。通过MTT法细胞生长抑制实验检测以及电镜下膜层表面细胞形态观察,发现nHA膜层比nHA/CTS和mHA/CTS能更有效地抑制胶质瘤细胞的增殖,具有明显的体外抗肿瘤作用。
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| 关 键 词： | 羟基磷灰石 壳聚糖 胶质母细胞瘤 |
The inhibitory effect on glioblastoma cells viability by nano-HA coatings and HA/CTS coatings |
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| TIAN Ang,WANG Chao,GUAN Ge-fei,XUE Xiang-xin,WU An-hua,WANG Li.The inhibitory effect on glioblastoma cells viability by nano-HA coatings and HA/CTS coatings[J].Journal of Functional Materials,2010,41(2). |
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| Authors: | TIAN Ang WANG Chao GUAN Ge-fei XUE Xiang-xin WU An-hua WANG Li |
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| Affiliation: | 1.School of Materials and Metallurgy;Northeastern University;Shenyang 110004;China;2.Departments of Neurosurgery;The First Affiliated Hospital;China Medical University;Shenyang 110001;China |
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| Abstract: | In current study,nano-hydroxyapatite coatings(nHA),nano-hydroxyapatite and micro-hydroxyapatite/chitosan complex films(nHA/CTS,mHA/CTS) were electrodeposited on the titanium substrate.The physical and chemical characteristics of the coatings were profiled using X-ray diffraction(XRD),SEM and FTIR determination.After co-cultured with human glioblastoma multiforme cell line U87(U87),the inhibitory effects of 3 kinds of surface layers on U87 cells viability were compared by MTT method and observation of the U8... |
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| Keywords: | hydroxyapatite chitosan glioma |
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