Calcium ions enclosed in casein phosphopeptide aggregates are directly involved in the mineral uptake by differentiated HT-29 cells |
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| Authors: | Stefania Cosentino Bianca Maria Donida Emiliano Marasco Elena Del Favero Laura Cantù Giovanni Lombardi Alessandra Colombini Stefania Iametti Serenella Valaperta Amelia Fiorilli Guido Tettamanti Anita Ferraretto |
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| Affiliation: | 1. Dipartimento di Chimica, Biochimica e Biotecnologie per la Medicina, Università degli Studi di Milano, Milano, Italy;2. IRCCS Istituto Ortopedico Galeazzi, Milano, Italy;3. Sezione di Biochimica, DISMA, Università degli Studi di Milano, Milano, Italy;4. IRCCS Istituto Clinico Humanitas, Rozzano, Milano, Italy;5. IRCCS Policlinico San Donato, San Donato Milanese, Milano, Italy;1. Department of Agricultural Sciences, University of Naples “Federico II”, 80055 Portici (Naples), Italy;2. Institute of Food Science, National Research Council, 83100 Avellino, Italy;3. Department of Neuroscience and Reproductive and Stomatological Sciences, University of Naples “Federico II”, 80131 Naples, Italy;1. IRCCS Istituto Ortopedico Galeazzi, Laboratory of Experimental Biochemistry & Molecular Biology, via R. Galeazzi 4, 20161 Milan, Italy;2. Dipartimento di Scienze per gli Alimenti, la Nutrizione e l''Ambiente, Università degli Studi di Milano, via G. Celoria 2, 20133 Milan, Italy;3. Dipartimento di Scienze Biomediche per la Salute, Università degli Studi di Milano, LITA, via Fratelli Cervi 93, 20090 Segrate, Italy;1. Istituto di Scienze dell’Alimentazione (ISA), CNR, Via Roma 64, 83100 Avellino, Italy;2. Dipartimento di Scienza degli Alimenti, Università di Napoli “Federico II”, Parco Gussone, Portici (NA) 80055, Italy;3. European Laboratory for the Investigation of Food Induced Diseases (ELFID), Università di Napoli “Federico II”, Napoli, Italy;4. Dipartimento di Scienze Agrarie, degli Alimenti e dell’Ambiente, Università di Foggia, Via Napoli 25, 71100 Foggia, Italy |
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| Abstract: | Casein phosphopeptides (CPPs) display the ability to form aggregates with calcium and promote the mineral uptake in models of intestinal cells. The present study aimed to clarify which fraction of calcium, CPPs – bound or free, is directly involved. A commercial preparation of calcium enriched CPPs was employed. The free intracellular calcium was monitored at the single cell level by fura-2 fluorescence. Results showed that calcium enriched CPPs formed aggregates and promoted mineral uptake by the cells at a stable level regardless of the amount of extracellular calcium, indicating that the fraction of calcium bound to CPPs was responsible of the bioactivity. The decalcified CPPs bind free calcium available in solution, form aggregates and promote the mineral uptake by the cells, but are dependent on extracellular calcium. |
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