固相包被技术及应用

对免疫分析中的生物分子包被固相载体技术做了系统的研究,并探索其在免疫分析中的应用。采用物理吸附法和多聚赖氨酸活化管化学连接方法对多克隆抗体和单克隆抗体在聚苯乙烯试管上的固定方法进行了研究,并用商品化的免疫分析试剂盒对研究的方法进行了评估。采用物理吸附方法包被的试管稳定性较好,应用于TSH和CA125免疫放射分析,技术指标均达到国家暂行标准。采用活化管技术包被的试管在应用某些具体的免疫分析试剂盒时,部分技术指标高于核行业标准,但需进一步的实验。

Technology of Antibody Immobilization on Solid Phase and its' Application in Immunoassay(In Chinese)

The technology of biomolecule immobilization on solid phase materials is studied and its' application in immunoassay is exploited. The polystyrene tubes were treated with poly-lysine to make the surface functionalized. The poly- or mono-clonal antibodies were immobilized on the surface-functionalized polystyrene tubes via covalent attachment. The antibody coated tubes were also prepared by passive adsorption. The antibody-attached tubes prepared with the two kind of procedure were evaluated with respect to their application in immunoassay. The passive adsorption procedure for antibody coating on polystyrene tubes was established. The antibodies coated on the tubes by the procedure were stable in radioimmunoassay and the two IRMA kits for TSH and CA125 using the antibody-coated tubes were developed. The performance characteristics of the two new kits reached National RIA standard. The technique of covalent attachment of antibody to poly-lysine surface-fictionalized polystyrene tubes was developed and used in radioimmunoassay, some performance characteristics are better than that in nuclear standard, but more study for its' use in RIA kits are needed.