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| 纤溶酶液的脱色及纯化研究 | |
| 引用本文： | 李宝库,周岩,马珦玻,崔哲.纤溶酶液的脱色及纯化研究[J].中国酿造,2007(7):25-27. |
| 作者姓名： | 李宝库 周岩 马珦玻 崔哲 |
| 作者单位： | 1. 河北大学,药学院,河北,保定,071002 2. 河北大学,附属医院,河北,保定,071001 3. 河北大学,医学部,河北,保定,071002 |
| 摘 要： | 以活性炭、高岭土以及D290和D392离子交换树脂为脱色介质,应用硫酸铵分级盐析以及超滤脱盐的方法,对纤溶酶液进行脱色及纯化。结果表明,D290强碱性阴离子交换树脂脱色效果优于D392弱碱性阴离子交换树脂,活性炭及高龄土不能对粗纤溶酶液脱色。70%饱和度的硫酸铵,工作压力0．11MPa,超滤脱盐纯化倍数达到12．37,冻干酶粉的比活力为638．9U/mg,回收率为73．37%。 |
| 关 键 词： | 纤溶酶液 离子交换 盐析 脱色 纯化 |
| 文章编号： | 2054-0571（2007）07-0025-03 |
| 修稿时间： | 2006-12-14 |
Decoloration and purification of fibrinolytic enzyme produced by microbial fermentation |
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| LI Bao-ku,ZHOU Yan,MA Xiang-bo,CUI Zhe.Decoloration and purification of fibrinolytic enzyme produced by microbial fermentation[J].China Brewing,2007(7):25-27. | |
| Authors: | LI Bao-ku ZHOU Yan MA Xiang-bo CUI Zhe |
| Affiliation: | 1. College of Pharmaceutical, Hebei Universit, Baoding 071002, China; 2. Aftfliated Hospital of Hebei University, Baoding 071000, China; 3. Health Science Center, Hebei University, Baoding 071002, China |
| Abstract: | The decoloration and purification of fibrinolytic enzyme were studied using salt fractionation with ammonium sulphate and ultrafitra- tion.Activated carbon,kaolin,ion exchange resin D290 and D392 were used as medium for decoloration and purification.The result showed that anion exchange resin D290 was more effective than D392 to remove color substances from the crude fibrinolytie enzyme.Activated car- bon and kaolin could not remove color substances.The purification fold was 12.37,the specific activity was 638.9 U/mg and the recovery was 73.37% under the conditions of 70% ammonium sulfate and 0.11 MPa pressure. |
| Keywords: | fibrinolytic enzyme ion exchange salt fractionation decoloration purification |
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