Identification of triacylglycerols in the enzymatic transesterification of rapeseed and butter oil |
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Authors: | Julia Svensson Patrick Adlercreutz |
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Affiliation: | Department of Biotechnology, Lund University, Lund, Sweden |
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Abstract: | A blend of rapeseed and butter oil was transesterified using immobilized Thermomyces lanuginosus lipase (Lipozyme® TL IM) as catalyst. The reaction was followed by reversed-phase HPLC and the triacylglycerol peaks were tentatively identified from their elution times by calculating equivalent carbon numbers. Further identification was made using HPLC-electrospray tandem mass spectrometry. A few of the triacylglycerols detected were typical combinations of fatty acids originating from rapeseed oil, such as α-linolenic acid, and short-chain fatty acids from butter oil. Due to the regioselectivity of the lipase, the transesterification reaction involved mainly fatty acids in the sn-1 and sn-3 positions. However, significant changes in the fatty acid composition in the sn-2 position were detected after 6 h. |
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Keywords: | HPLC-electrospray tandem mass spectrometry Lipozyme TL?IM Reversed-phase HPLC Transesterification Triacylglycerols |
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