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Influences of electroosmotic flows in nanopillar chips on DNA separation: Experimental results and numerical simulations |
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| Authors: | Noritada Kaji Akio Oki Ryo Ogawa Yuzuru Takamura Takahiro Nishimoto Hiroaki Nakanishi Yasuhiro Horiike Manabu Tokeshi Yoshinobu Baba |
| Affiliation: | 1. Department of Applied Chemistry, Graduate School of Engineering, Nagoya 464-8603, Japan;2. Biomaterials Center, National Institute for Materials Science, Ibaraki 305-0044, Japan;3. Department of Chemical Materials Science, School of Materials Science, Japan Advanced Institute of Science and Technology, Ishikawa 923-1292, Japan;4. Technology Research Laboratory, Shimadzu Corporation, Kyoto 619-0237, Japan;5. Department of Applied Chemistry, Graduate School of Engineering, Nagoya 464-8603, Japan
MEXT Innovative Research Center for Preventive Medical Engineering, Nagoya 464-8603, Japan;6. Department of Applied Chemistry, Graduate School of Engineering, Nagoya 464-8603, Japan MEXT Innovative Research Center for Preventive Medical Engineering, Nagoya 464-8603, Japan Plasma Nanotechnology Research Center, Nagoya University, Nagoya 464-8603, Japan Health Technology Research Center, National Institute of Advanced Industrial Science and Technology (AIST), Takamatsu 761-0395, Japan |
| Abstract: | The various potential factors affecting the performance of nanopillar chips on DNA separation were investigated from the viewpoints of both numerical calculations and actual experiments. To yield higher performance and replace the conventional DNA separation techniques such as microchip electrophoresis, the phenomenon specific to the nanopillar chips should be deeply understood. In this paper, although various factors affecting the performance of the nanopillar chips are considered, we focused on the effect of electroosmotic flow, which is particularly noticeable in quartz-made nanopillar chips. High-resolution separation of DNA was realized when an electroosmotic flow was suppressed by simply using a higher concentration of buffer, but DNA separation failed in the presence of an electroosmotic flow. It was confirmed from the numerical simulations and the direct observations that the deformation of DNA band during the injection process was induced by electroosmotic flow and consequently led to a poor resolution. |
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