Multivariate model for the prediction of soluble condensed tannins in crude extracts of polyphenols from canola and rapeseed hulls |
| |
Authors: | M Naczk J Pink R Amarowicz D Pink F Shahidi |
| |
Affiliation: | (1) Food Research Laboratory, St. Francis Xavier University, B2G 2W5 Antigonish, Nova Scotia, Canada;(2) Division of Food Science, Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Olsztyn, Poland;(3) Department of Biochemistry, Memorial University of Newfoundland, A1B 3X9 St. John’s, Newfoundland, Canada;(4) Department of Human Nutrition, St. Francis Xavier University, P.O. Box 5000, B2G 2W5 Antigonish, NS, Canada |
| |
Abstract: | The feasibility of using ultraviolet spectrophotometry to develop multivariate models for prediction of soluble condensed
tannins (SCT) content in crude polyphenols extracts from canola and rapeseed hulls was investigated. The polyphenols were
extracted from hulls using 70% (vol/vol) aqueous acetone. Partial least squares regression was used to correlate the spectral
data of the crude polyphenols in methanol between 265–295 nm with the SCT content in hulls. Both the proanthocyanidin (P)
and the vanillin (V) assays were used to provide reference data for creating the models. The predictive ability of the models
is good, as indicated by the RPD values the ratio of the standard deviation of data to the standard error of calibration
(SEC) of above 5. Additionally, the SEC values suggest that P is superior to V in predicting the SCT content of hulls using
this method. |
| |
Keywords: | Canola hulls PLS rapeseed soluble condensed tannins UV spectroscopy |
本文献已被 SpringerLink 等数据库收录! |
|