Increased response of Vero cells to PHBV matrices treated by plasma |
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Authors: | Carolina Lucchesi Betina M P Ferreira Eliana A R Duek Jr" target="_blank">Arnaldo R SantosJr Paulo P Joazeiro |
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Affiliation: | (1) Department of Histology and Embryology, Institute of Biology, State University of Campinas (UNICAMP), PO Box 6109, Campinas, 13083-970, SP, Brazil;(2) Department of Physiological Sciences, Faculty of Biological Sciences, Pontifical Catholic University of Sao Paulo (PUC-SP), Sorocaba, 18030-230, SP, Brazil;(3) Department of Cell Biology, State University of Campinas (UNICAMP), PO Box 6109, Campinas, 13083-970, SP, Brazil |
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Abstract: | The copolymers poly(3-hydroxybutyric acid-co-3-hydroxyvaleric acid) (PHBV) are being intensely studied as a tissue engineering
substrate. It is known that poly 3-hydroxybutyric acids (PHBs) and their copolymers are quite hydrophobic polyesters. Plasma-surface
modification is an effective and economical surface treatment technique for many materials and of growing interest in biomedical
engineering. In this study we investigate the advantages of oxygen and nitrogen plasma treatment to modify the PHBV surface
to enable the acceleration of Vero cell adhesion and proliferation. PHBV was dissolved in methylene chloride at room temperature.
The PHBV membranes were modified by oxygen or nitrogen-plasma treatments using a plasma generator. The membranes were sterilized
by UV irradiation for 30 min and placed in 96-well plates. Vero cells were seeded onto the membranes and their proliferation
onto the matrices was also determined by cytotoxicity and cell adhesion assay. After 2, 24, 48 and 120 h of incubation, growth
of fibroblasts on matrices was observed by scanning electron microscopy (SEM). The analyses of the membranes indicated that
the plasma treatment decreased the contact angle and increased the surface roughness; it also changed surface morphology,
and consequently, enhanced the hydrophilic behavior of PHBV polymers. SEM analysis of Vero cells adhered to PHBV treated by
plasma showed that the modified surface had allowed better cell attachment, spreading and growth than the untreated membrane.
This combination of surface treatment and polymer chemistry is a valuable guide to prepare an appropriate surface for tissue
engineering application. |
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