| HPLC法测定归芍护肝颗粒中芍药苷的含量 |
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| 引用本文： | 戴芸,胡秀,杨林,柯春霞. HPLC法测定归芍护肝颗粒中芍药苷的含量[J]. 当代化工, 2016, 0(3): 476-478. DOI: 10.3969/j.issn.1671-0460.2016.03.013 |
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| 作者姓名： | 戴芸 胡秀 杨林 柯春霞 |
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| 作者单位： | 黄石市中心医院 湖北理工学院附属医院，湖北 黄石 435000; 肾脏疾病发生与干预湖北省重点实验室，湖北 黄石 435000 |
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| 摘 要： | 建立测定归芍护肝颗粒中芍药苷含量的方法。采用HPLC-UV法,Aglient ZORBAX SB-C18柱(4.6×250 mm,5μm);流动相为乙腈-0.1%磷酸溶液(14:86,v/v)为流动相;检测波长为230 nm;柱温为30℃;流速为1 m L/min。芍药苷在10~100μg/m L范围内线性关系良好,平均回收率为98.79%,RSD分别为0.92%。该法简便、准确、灵敏、重现性好,可用于归芍护肝颗粒中芍药苷含量的测定。
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| 关 键 词： | HPLC 归芍护肝颗粒 芍药苷 含量测定 |
Determination of Peoniflorin Content in Guishao Hugan Granules by HPLC |
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| Abstract: | A method for determination of peoniflorin content in Guishao Hugan Granules was established. A HPLC-UV method was developed. The separation was performed on a ZORBAX SB-C18 column (4.6×250 mm, 5 μm). The mobile phase consisted of acetonitrile- 0.1% phosphoric acid (14:86, v/v) at the flow rate of 1.0 mL/min. The detection wavelength was 230 nm. The column temperature was 30 ℃. The calibration curves of peoniflorin showed good linearity in the range of 10~100 μg/mL. The average recoveries were 98.79% with RSD of 0.92%. The method is simple, accurate, sensitive and reproducible for determination of peoniflorin content in Guishao Hugan Granules. |
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| Keywords: | HPLC Guishao hugan granules Peoniflorin Content determination |
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