短期摄食卵黄磷脂与鱼油对高脂小鼠脂质代谢的影响

探究马尾松花粉提取物(MPPE)对前列腺肥大(BPH)小鼠的影响。雄性昆明种小鼠随机分为6组，分别为空白对照组、BPH组、20、40和80 mg/kg?d MPPE组及非那雄胺(0.8 mg/kg?d)组。预先灌胃MPPE两周之后，在腹腔注射丙酸睾酮建模的同时连续灌胃MPPE或非那雄胺四周。实验结束后观察组织形态变化，测定细胞因子IL-1β、IL-6、TNF-α、PACP、DHT、NO及NOS的水平。利用UPLC-QTOF-MS技术检测小鼠血清、尿液中代谢物的变化，采用PLS-DA分类，寻找潜在生物标志物。三种剂量的MPPE处理均降低了前列腺指数、炎性细胞因子、DHT浓度及PACP、NOS的活性，并减少了NO的生成。血清代谢组学鉴定了3个潜在生物标记物，分别为1-棕榈酰溶血磷脂酰胆碱、1-O-十六烷基-2-O-乙酰基-sn-甘油基-3-磷酸胆碱和(Z)-13-二十二烯酰胺。马尾松花粉提取物可以明显降低BPH病理症状及相关生化指标，调节标志性代谢物的水平。其可能的作用机制与抑制炎症及缓解脂质代谢紊乱相关。

Effects of Short-term Consumption of Fish Oil-egg-phospholipid on Lipid Metabolism in Mice Fed a High-fat Diet

The effects of short-term consumption of docosahexaenoic acid (DHA)-enriched phospholipids, DHA-enriched triglycerides, and the combination of DHA-enriched triglycerides and egg-phospholipids on lipid metabolism in mice fed a high-fat diet were compared. C57BL/6J mice were divided into five groups: low-fat group, high-fat group, DHA-enriched phospholipid group, DHA-enriched triglyceride group, and DHA-enriched triglyceride-egg-phospholipids combination group. Mice were fed the high-fat diet for a week; thereafter, lipid levels in the serum, liver, and brain were measured, along with the fatty acid compositions in the liver and brain. The short-term dietary supplementation of DHA could improve lipid metabolism in mice to different degrees. The serum triglyceride (TG) concentration was significantly reduced by DHA-enriched phospholipids and by the combination of DHA-enriched triglycerides and egg-phospholipids to the same degree. DHA-enriched phospholipids were superior to the combination of DHA-enriched triglycerides and egg-phospholipids in decreasing total serum cholesterol. The hepatic TG level in the combination group was notably lower than that in the high-fat group. However, no significant difference was observed in the hepatic TG level between the DHA-enriched phospholipids group and the combination group. Furthermore, DHA-enriched phospholipids and the combination of DHA-enriched triglycerides and egg-phospholipids could significantly increase the content of DHA in the liver and brain. DHA-enriched phospholipids could more effectively improve lipid metabolism in the serum and liver of mice fed a high-fat diet, and showed the highest increase in brain DHA level.