藻胆蛋白在大肠杆菌中的重组表达及其光谱学性质与抗氧化活性研究

研究发现从红藻坛紫菜中提取的多糖具有优异的抗氧化活性。本文研究了坛紫菜多糖及其硫酸衍生物对两种突变剂环磷酰胺和丝裂霉素C诱导的大蒜根尖细胞微核率、有丝分裂指数和染色体畸变率的影响作用，以及抗脂质过氧化作用。结果表明，两种多糖均表现出明显地抑制微核和染色体畸变作用，明显地提高有丝分裂指数。其影响作用具有明显的剂量依赖性。当多糖浓度为100 mg/L时，多糖表现出最大抑制效应或增强效应。同时，硫酸化衍生物表现出比原料多糖更优异的抗细胞内脂质过氧化作用。分析表明坛紫菜多糖表现出的抗突变作用与抗脂质过氧化作用具有一定关系。同时也表明硫酸基对于抗突变作用的间接影响。结果显示坛紫菜多糖具有成为抗突变活性物质的潜力。

Optimization of Expression of Recombinant Phycobiliproteins in Escherichia coli and Assessment of Their Spectral Properties and Antioxidant Activity

Studies have shown that porphyran extracted from the red algae Porphyra haitanesis has a potential antioxidant activity. The effect of porphyran and its sulfate derivatives on cyclophosphamide (CP)- and mitomycin C (MMC)-induced changes in micronucleus rate, mitotic index, chromosome aberration rate, and lipid peroxidation in Allium sativum root cells was investigated. The results indicated that the polysaccharide significantly inhibited the micronucleus rate and chromosomal aberration rate, and significantly enhanced the mitotic index in a dose dependent manner. The polysaccharide exhibited the highest inhibitory and enhancing effects at 100 mg/L concentration. Additionally, the sulfate derivative exhibited superior resistance towards lipid peroxidation in cells compared to that exhibited by the unmodified polysaccharide. The analysis reveals that the anti-carcinogenic effect of porphyran has a definite relationship with anti-lipid peroxidation activity, and shows that the sulfate group has an indirect effect on anti-carcinogenic activity. The results showed that porphyran has an anti-carcinogenic potential.