Laser Vaporization/Ionization Interface for Capillary Electrophoresis-Time-of-Flight Mass Spectrometry |
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Authors: | Chang S Y Yeung E S |
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Affiliation: | Ames Laboratory [Formula: see text] USDOE and Department of Chemistry, Iowa State University, Ames, Iowa 50011. |
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Abstract: | Mass spectrometry (MS) is usually coupled on-line with capillary electrophoresis (CE) to analyze biomolecules by using electrospray ionization or continuous-flow fast-atom bombardment. We present a new design for laser vaporization/ionization time-of-flight mass spectrometry. CE, with its low flow rate (<1 μL/min), is highly compatible with MS, even if the total column effluent is introduced directly. A UV laser is used to vaporize and ionize the solution eluting from the column. There is no need to have a makeup solvent. Using this system, we have analyzed a group of amines and peptides. The concentration detection limit of serotonin is in the 10(-)(7) M level. The separation and identification of an amine mixture by CE/MS demonstrates the complementary nature of the information. |
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