| 不同栽培模式杜仲体绿原酸含量积累动态的研究 |
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| 引用本文： | 杨秀芳,汪洋,马养民.不同栽培模式杜仲体绿原酸含量积累动态的研究[J].西北轻工业学院学报,2012(2):9-11. |
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| 作者姓名： | 杨秀芳 汪洋 马养民 |
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| 作者单位： | 陕西科技大学教育部轻化工助剂化学与技术重点实验室 |
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| 摘 要： | 研究杜仲绿原酸积累的动态规律,为确定杜仲合理栽培模式和最佳采收期提供依据.定期定点采集乔林栽培和叶林栽培的每年4～10月杜仲叶、皮样品,采用高效液相色谱（HPLC）法,分析杜仲体中绿原酸的含量.结果表明杜仲体中绿原酸动态积累有一定规律,叶林模式栽培的杜仲叶中绿原酸含量最高,且在6月达到全年最大值.实验结论：根据杜仲体中的绿原酸积累动态规律,杜仲的最佳栽培模式为叶林栽培,最佳采收期为6月.
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| 关 键 词： | 杜仲 栽培模式 绿原酸 积累动态 |
Research on the Accumulation Dynamic of Chlorogenic Acid in Eucommia Ulmoides Oliv Different Planting Patterns |
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| YANG Xiu-fang,WANG Yang,MA Yang-min.Research on the Accumulation Dynamic of Chlorogenic Acid in Eucommia Ulmoides Oliv Different Planting Patterns[J].Journal of Northwest University of Light Industry,2012(2):9-11. |
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| Authors: | YANG Xiu-fang WANG Yang MA Yang-min |
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| Affiliation: | (Key Laboratory of Auxiliary Chemistry & Technology for Chemical Industry,Ministry of Education,Shaanxi University of Science & Technology,Xi′an 710021,China) |
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| Abstract: | To study the extraction and separation technology of chlorogenic acid from Eucommia ulmoides leaves.Water extracting technology of chlorogenic acid was studied through the orthogonal test.Purification process of chlorogenic acid by column chromatography was discussed.The optimum technological parameters of water extraction were as followed: ratio of leaves to solvent was 1∶8,extracting temperature was 80℃,extracting times was twice,the leaves were extracted by hot water for 70 min.With this method,the extraction yield of chlorogenic acid could reach 1.78%.Chlorogenic acid could be purified by D-101 macroporous resin column chromatography,silica gel column chromatography and Sephadex LH-20 column chromatography.The content of chlorogenic acid in the leaves of leaf-oriented tree model was higher. |
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| Keywords: | Eucommia ulmoides Oliv cultivation pattern chlorogenic acid dynamic accumulation |
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