恒温荧光核酸检测仪检测2种食源性致病菌的方法验证

目的以传统国标培养法作为参考比对方法,考察恒温荧光核酸检测仪(constant temperature fluorescent nucleic acid detector)检测食品(自然食品和加标食品)中食源性致病菌如阪崎肠杆菌和单核细胞增生李斯特氏菌的一致性。方法通过对食品中阪崎肠杆菌和单核细胞增生李斯特氏菌国标培养法和恒温荧光核酸检测仪方法检测结果的比对,考量后者方法的灵敏度、特异性、假阳性率、假阴性率和准确度。结果恒温荧光核酸检测仪检测食品中阪崎肠杆菌和单核细胞增生李斯特氏菌的灵敏度和特异性都是100%,假阴性率为0;假阳性率为0。结论恒温荧光核酸检测仪方法特异性强、准确度高、无一例漏检。

Method verification for the detection of 2 kinds of food pathogens by constant temperature fluorescent nucleic acid detector

Objective To evaluate the consistency of the determination of foodborne pathogenic microorganisms such as Enterobacter sakazakii and Listeria monocytogenes by constant temperature fluorescent nucleic acid detector, with the traditional national standard culture method as a reference alignment method. Methods The sensitivity, specificity, false positive rate, false negative rate and accuracy of constant temperature fluorescent nucleic acid detector for Enterobacter sakazakii and Listeria monocytogenes were investigated with the national standards method as reference. Results There were no false negative and misdetection rate. Both the sensitivities and specificities of Enterobacter sakazakii and Listeria monocytogenes detected by the constant temperature fluorescent nucleic acid detector were 100%. Conclusion The constant temperature fluorescent nucleic acid detector method has the advantages of high specificity and accuracy, and has no missing detection.