Mechanism of oxidative phosphorylation. IV. Role of the closed state of mitochondrial membranes

The clousness of membrane of rat brain mitochondria isolated in Krebs-Ringer's solution was broken by suspending the mitochondria in distilled water. Function characteristics of lyzed organells did not decrease but were somewhat higher than those of the organells suspended in Krebs-Ringer's solution. The results were discussed in relation to the question of the role of clousness of the mitochondrial membrane in the mechanism of oxidative phosphorylation.