| 99mTc直接法标记抗体的3个常用还原剂的评价 |
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| 引用本文： | 张汝森,刘长征.99mTc直接法标记抗体的3个常用还原剂的评价[J].核技术,2000,23(5):312-315. |
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| 作者姓名： | 张汝森 刘长征 |
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| 作者单位： | [1]广州市红十字会医院,广州 [2]中山医科大学,广州 |
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| 摘 要： | 比较了＾９９ｍＴｃ直接法标记抗体的３个常用的还原剂：氯化亚锡（ＳｎＣｌ２），２－疏工乙醇（２－ＭＥ）和抗坏血酸（ＡＡ）的＾９９ｍＴｃ标记率、标记抗体的稳定性及免疫活性。结果显示，２－ＭＥ是一个良好的还原剂，当抗体／２－ＭＥ摩尔比为１：２０００时，抗体的标记率可达到９５％以上，胶体形成量少于５％，＾９９ｍＴｃ－ＢＡＣ５肿瘤放射免疫显像，鼻咽癌转移癌影像清晰。
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| 关 键 词： | 同位素标记抗体 放射免疫显像 鼻咽癌 锝－99 |
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Reduction-mediated technetium-99m labeling of antibodies -evaluation of various reducing agents |
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| ZHANG Rusen, LIU Changzheng.Reduction-mediated technetium-99m labeling of antibodies -evaluation of various reducing agents[J].Nuclear Techniques,2000,23(5):312-315. |
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| Authors: | ZHANG Rusen LIU Changzheng |
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| Affiliation: | ZHANG Rusen1, LIU Changzheng2 1 |
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| Abstract: | Three common reducing agents: stannous chloride (SnCl_2), ascorbic acid (AA), 2-mercapoethanol (2-ME) have been compared for their efficacy of labeling antibodies(Abs) with ~(99m)Tc, stability and immunoreactivity of labeled Abs The results showed that 2-ME was an optimum reducing agent, at a molar ratio of 1:2000 for Ab to 2-ME. A ~(99m)Tc labeling efficiency greater than 95% can be obtained, the colloid formation was less than 5%. The radioimmunoimaging of ~(99m)Tc-BAC_5 in nude mice bearing tumor xenografts showed that tumor can be viewed clearly. |
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| Keywords: | Isotope labeling/method Antibody Technetium-99m Radioimmunoimaging |
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