| Synthesis,characterization r）oly [LA-co-（GIc-alt-Lys）] for and biological evaluation of nerve regeneration scaffold |
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| 作者姓名： | Yi-Xia YIN Ji-Ling YI Li-Juan XIE Qiong-Jiao YAN Hong-Lian DAI Shi-Pu LI |
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| 作者单位： | Biomedical Materials and Engineering Research Center, Wuhan University of Technology, Wuhan 430070, China |
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| 摘 要： | A novel nerve repairing material poly LA-co.（GIc-alt-Lys）] （PLGL） was synthesized. The viability and growth of Schwann cells （SCs） co-cultured With poly （D, L- lactic acid） （PDLLA） films （control group） and PLGL films were evaluated by MTT assay and SEM observation. Then, contact angle measurement, histological assessment and enzyme-linked immunosorbent assay （ELISA） testing on inflammatory-related cyto- kines such as IL-10 and TGF-β1 were performed. The results showed that, compared with PDLLA, PLGL films possesses better hydrophilicity, biocompatibility, degradation property and less inflammatory reaction. The present study indicated that PLGL scaffolds would meet the requirements of artificial nerve scaffold and have a potential application in the fields of nerve regeneration.
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| 关 键 词： | 神经再生 支架材料 赖氨酸 生物学评价 合成 酶联免疫吸附试验 表征 L-乳酸 |
Synthesis,characterization and biological evaluation of poly [LA-co-(Glc-alt-Lys)] for nerve regeneration scaffold |
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| Authors: | Yi-Xia Yin Ji-Ling Yi Li-Juan Xie Qiong-Jiao Yan Hong-Lian Dai Shi-Pu Li |
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| Affiliation: | Biomedical Materials and Engineering Research Center, Wuhan University of Technology, Wuhan 430070, China |
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| Abstract: | A novel nerve repairing material poly LA-co-(Glc-alt-Lys)] (PLGL) was synthesized. The viability and growth of Schwann cells (SCs) co-cultured with poly (D, Llactic acid) (PDLLA) films (control group) and PLGL films were evaluated by MTTassay and SEM observation. Then, contact angle measurement, histological assessment and enzyme-linked immunosorbent assay (ELISA) testing on inflammatory-related cytokines such as IL-10 and TGF-β1 were performed. The results showed that, compared with PDLLA, PLGL films possesses better hydrophilicity, biocompatibility, degradation property and less inflammatory reaction. The present study indicated that PLGL scaffolds would meet the requirements of artificial nerve scaffold and have a potential application in the fields of nerve regeneration. |
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| Keywords: | Schwann cell （SC） poly [LA-co-（GIc alt-Lys）] （PLGL） implantation inflammatory factor biocompatibility |
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