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Large-scale dendrimer-based uneven nanopatterns for the study of local arginine-glycine-aspartic acid (RGD) density effects on cell adhesion
作者姓名:Anna Lagunas  ;Albert G. Castano  ;Juan M. Artes  ;Yolanda Vida  ;Daniel Collado  ;Ezequiel Perez-lnestrosa  ;Pau Gorostiza  ;Silvia Claros  ;Jose A. Andrades  ;Josep Samitier
作者单位:[1]Networking Biomedical Research Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Spain; [2]Institute for Bioengineering of Catalonia (IBEC), Baldiri-Reixac 15-2 1, Barcelona 08028, Spain; [3]Physical Chemistry Department, University of Barcelona (UB), Martii Franques 1-11, Barcelona 08028, Spain; [4]Andalusian Centre for Nanomedicine and Biotechnology (BIONAND), Severo Ochoa 35, Mdlaga 29590, Spain; [5]Organic Chemistry Department, University of Mdlaga (UMA), Campus Teatinos, Malaga 29071, Spain; [6]Institucio Catalana de Recerca i Estudis Avanats (ICREA), Spain; [7]Cell Biology, Genetics and Physiology Department, University of Malaga (UMA), Campus Teatinos, Malaga 29071, Spain; [8]Electronics Department, University of Barcelona (UB), Martii Franques 1-11, Barcelona 08028, Spain t; [9]Present address: Electrical and Computer Engineering Department, University of California Davis, 95616 Davis CA, USA; [10]7.1, University of California Davis, 95616 Davis CA, USA;
基金项目:The authors thank Professor A. Hari Reddi for fruitful discussions and M. Ldpez for help in STM measurements. The authors also thank M. Sanmarti for help in zeta potential measurements. This work was supported by Centro de Investigacion Biomedica en Red en Bioingenierla, Biomateriales y Nanomedicina (CIBER-BBN), Spain. The Nanobioengineering group at the Institute for Bioengineering of Catalonia (IBEC) receives support from the Commission for Universities and Research of the Department of Innovation, Universities and Enterprise of the Generalitat de Catalunya (No. 2009 SGR 505). In addition, research leading to these results received support from the Spanish Ministry of Science and Education; Ministry of Economy and Competitiveness (Red TerCel; FIS PI10/02529); the Andalusian Government (Nos. P07- CVI-2781, PAIDI BIO-217, PI-0729-2010); Fundacion Botln, Santander, Spain; CICYT (Nos. CTQ2009-07758; CTQ2010-20303); Commission for Universities and Research of the Department of Innovation, Universities and Enterprise of the Generalitat de Catalunya (2009 SGR 505).
摘    要:Cell adhesion processes are governed by the nanoscale arrangement of the extracellular matrix (ECM), being more affected by local rather than global concentrations of cell adhesive ligands. In many cell-based studies, grafting of dendrimers on surfaces has shown the benefits of the local increase in concentration provided by the dendritic configuration, although the lack of any reported surface characterization has limited any direct correlation between dendrimer disposition and cell response. In order to establish a proper correlation, some control over dendrimer surface deposition is desirable. Here, dendrimer nanopatterning has been employed to address arginine-glycine-aspartic acid (RGD) density effects on cell adhesion. Nanopatterned surfaces were fully characterized by atomic force microscopy (AFM), scanning tunneling microscopy (STM) and X-ray photoelectron spectroscopy (XPS), showing that tunable distributions of cell adhesive ligands on the surface are obtained as a function of the initial dendrimer bulk concentration. Cell experiments showed a clear correlation with dendrimer surface layout: Substrates presenting regions of high local ligand density resulted in a higher percentage of adhered cells and a higher degree of maturation of focal adhesions (FAs). Therefore, dendrimer nano- patterning is presented as a suitable and controlled approach to address the effect of local ligand density on cell response. Moreover, due to the easy modification of dendrimer peripheral groups, dendrimer nanopatterning can be further extended to other ECM ligands having density effects on cells.

关 键 词:树枝状聚合物  细胞粘附  密度效应  天冬氨酸  精氨酸  甘氨酸  RGD  X射线光电子能谱

Large-scale dendrimer-based uneven nanopatterns for the study of local arginine-glycine-aspartic acid (RGD) density effects on cell adhesion
Anna Lagunas,;Albert G. Castano,;Juan M. Artes,;Yolanda Vida,;Daniel Collado,;Ezequiel Perez-lnestrosa,;Pau Gorostiza,;Silvia Claros,;Jose A. Andrades,;Josep Samitier.Large-scale dendrimer-based uneven nanopatterns for the study of local arginine-glycine-aspartic acid (RGD) density effects on cell adhesion[J].Nano Research,2014,7(3):399-409.
Authors:Anna Lagunas  Albert G Castaño  Juan M Artés  Yolanda Vida  Daniel Collado  Ezequiel Pérez-Inestrosa  Pau Gorostiza  Silvia Claros  José A Andrades  Josep Samitier
Affiliation:1. Networking Biomedical Research Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Badajoz, Spain
2. Institute for Bioengineering of Catalonia (IBEC), Baldiri-Reixac 15-21, Barcelona, 08028, Spain
3. Physical Chemistry Department, University of Barcelona (UB), Martí i Franquès 1-11, Barcelona, 08028, Spain
4. Andalusian Centre for Nanomedicine and Biotechnology (BIONAND), Severo Ochoa 35, Málaga, 29590, Spain
5. Organic Chemistry Department, University of Málaga (UMA), Campus Teatinos, Málaga, 29071, Spain
6. Institució Catalana de Recerca i Estudis Avan?ats (ICREA), Barcelona, Spain
7. Cell Biology, Genetics and Physiology Department, University of Málaga (UMA), Campus Teatinos, Málaga, 29071, Spain
8. Electronics Department, University of Barcelona (UB), Martí i Franquès 1-11, Barcelona, 08028, Spain
Abstract:Cell adhesion processes are governed by the nanoscale arrangement of the extracellular matrix (ECM), being more affected by local rather than global concentrations of cell adhesive ligands. In many cell-based studies, grafting of dendrimers on surfaces has shown the benefits of the local increase in concentration provided by the dendritic configuration, although the lack of any reported surface characterization has limited any direct correlation between dendrimer disposition and cell response. In order to establish a proper correlation, some control over dendrimer surface deposition is desirable. Here, dendrimer nanopatterning has been employed to address arginine-glycine-aspartic acid (RGD) density effects on cell adhesion. Nanopatterned surfaces were fully characterized by atomic force microscopy (AFM), scanning tunneling microscopy (STM) and X-ray photoelectron spectroscopy (XPS), showing that tunable distributions of cell adhesive ligands on the surface are obtained as a function of the initial dendrimer bulk concentration. Cell experiments showed a clear correlation with dendrimer surface layout: Substrates presenting regions of high local ligand density resulted in a higher percentage of adhered cells and a higher degree of maturation of focal adhesions (FAs). Therefore, dendrimer nanopatterning is presented as a suitable and controlled approach to address the effect of local ligand density on cell response. Moreover, due to the easy modification of dendrimer peripheral groups, dendrimer nanopatterning can be further extended to other ECM ligands having density effects on cells.  src=
Keywords:dendrimer  arginine-glycine-asparticacid  atomic force microscopy  scanning tunnelingmicroscopy  cell adhesion  focal adhesions
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