| 制革污泥中产脂肪酶真菌的筛选及产酶条件优化 |
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| 引用本文： | 马宏瑞,罗茜,朱超.制革污泥中产脂肪酶真菌的筛选及产酶条件优化[J].西北轻工业学院学报,2013(6):25-30. |
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| 作者姓名： | 马宏瑞 罗茜 朱超 |
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| 作者单位： | 陕西科技大学资源与环境学院,陕西西安710021 |
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| 基金项目： | 国家自然科学基金项目（21177079）;陕西科技大学博士科研启动基金项目（BJ12-29）;陕西科技大学研究生创新基金项目： |
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| 摘 要： | 通过利用罗丹明B培养基和溴甲酚紫培养基初筛和酶活的测定法复筛，从制革厂含铬污泥（2．77mg／L）中筛选出一株产脂肪酶的真菌，经形态学观察、生理生化测定和分子生物学鉴定，初步鉴定该株菌为子囊菌门煤炱目下Teratosphaeriaceae科的一种，暂定名为Teratosphaneriacen8Crl2．同时对该菌株的摇床培养产酶条件进行了初步优化，得到最佳发酵条件为初始PH值为5．5，培养温度为35℃，种龄18h，接种量为2．5％（v／V），发酵周期为72h，转速为150r／min，酶活力最高达到8．5U／mL．
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| 关 键 词： | 制革污泥 脂肪酶 真菌 筛选 发酵 优化 |
Screening of fungi producing lipase from tanning sludge and optimization of the lipase production |
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| Affiliation: | MA Hong-rui, LUO Xi, ZHU (College of Resource and Environment, Shaanxi University of Science Xi＇an 710021, China) |
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| Abstract: | aeriaceae under Capnodi ales of Ascomycota, was screened from tanning sludge in south China by the color agar plate method, morphological observation, determination of enzyme activity, physiological and bi- ochemical determination and molecular biological identification, and was nominated as Ter- atosphaeriaceae Crl2. Preliminary optimization of the shaker-based fermentation conditions of the lipase from Teratosphaeriaceae Cr12 were determined. The optimum fermentation conditions for enzyme production were as follows, initial pH value as 5.5, fermentation tem- （V/V） , fermenta- the lipase activity |
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| Keywords: | tanning sludge lipase fungi screening fermentation optimizati |
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