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米曲霉及激光诱变菌株间的同工酶亲缘关系研究
引用本文:向洋,朱泽瑞.米曲霉及激光诱变菌株间的同工酶亲缘关系研究[J].高技术通讯,1997,7(3):12-16.
作者姓名:向洋  朱泽瑞
作者单位:[1]长沙电力学院生物系 [2]湖南师范大学生物系
摘    要:报道了我国酱油酿造菌米曲霉的8个原始菌株以及11个经筛选、提纯复壮的菌株和激光诱变菌株的酯酶同工酶和淀粉酶同工酶的研究。结果表明:米曲霉酯酶同工酶分11个带区,有4个带区通常分别有2条酶带出现。米曲霉淀粉同工酶一般有4条酶带,但有的菌株只有2条酶带,两种同工酶谱均显示出各原始菌株间酶带的差异,表明了菌株来源的差异。有3株诱变株的酶带变化很大,表明通过诱变发生了变异,筛选株与原始株间几乎无酶谱变化。

关 键 词:米曲霉  激光诱变  亲缘关系  酱油  同工酶

Investigations of Relationship among Aspergillus Oryza Strains by Isoenzyme Electrophoresis
Xiang Yang, Zhu Zerui, Gong Sui.Investigations of Relationship among Aspergillus Oryza Strains by Isoenzyme Electrophoresis[J].High Technology Letters,1997,7(3):12-16.
Authors:Xiang Yang  Zhu Zerui  Gong Sui
Abstract:This paper reports the study of esterase and amylase isoenzyme of Aspergillus Oryza which has 8 original strains and 11 screened strains after being purified or induced by haser. The results Show that Aspergillus Dryza esterase isoenzyme generally has 11 hand zones, and 4 of which usually respectively have 2 enzyme bands, while the amylase isoenzyme generally has 4 band zones, but in some of the Sirius there are only 2 bsnds. There is difference among the isoenzyme bsnd of the original strains, whiCh illdicates differences of the source of strains. There is variance of enzyme bands in 3 induced strains, which shows they have been variated by the inducement. There are no clear difference between the original and the screened strains. The esterase isoenzyme's band is more complicated and varied in contrast to the hands of the two isoenzymes. It is suitable for the study of its origin.
Keywords:Aspergillus Dryza  Esterase isoenzyme  Amylase isoenzyme  Relationship  Laser biology
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