Growth of immobilized DNA by polymerase: bridging nanoelectrodes with individual dsDNA molecules |
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Authors: | Linko Veikko Leppiniemi Jenni Shen Boxuan Niskanen Einari Hytönen Vesa P Toppari J Jussi |
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Affiliation: | Nanoscience Center, Department of Physics, University of Jyv?skyl?, P.O. Box 35, FI-40014, Jyv?skyl?, Finland. veikko.linko@jyu.fi |
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Abstract: | We present a method for controlled connection of gold electrodes with dsDNA molecules (locally on a chip) by utilizing polymerase to elongate single-stranded DNA primers attached to the electrodes. Thiol-modified oligonucleotides are directed and immobilized to nanoscale electrodes by means of dielectrophoretic trapping, and extended in a procedure mimicking PCR, finally forming a complete dsDNA molecule bridging the gap between the electrodes. The technique opens up opportunities for building from the bottom-up, for detection and sensing applications, and also for molecular electronics. |
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