壳聚糖涂层亲和层析在超氧化物歧化酶纯化中的应用

1前言亲和层析是蛋白质纯化中分离效率最高的技术之一。亲和配基有多种形式如单机、底物、活性染料、金属离子等。固定化金属离子亲和层析（Immobilizedmetalionaffinitychromatog’“PhyIMAC）是Porath在1975年首先研究的一种高效分离技术［‘3。IMAC和普通的亲和层析相比,具有一些优点,如亲和配基Cu’”、Zn’“价廉;可在高盐浓度下操作;稳定,容易再生。Sulkowke曾用IMAC技术分离纯化干扰素,一步可纯化产品几十倍【“,收率达90％。目前IMAC介质制备是先在软基质SePharose或SePhadex上用化学交联法弓卜螫合剂IDA（Imino…

Purification of Superoxide Dismutase with Chitosan Coated Affinity Adsorbent

A new adsorbent for immobilized metal ion affinity chromatography (IMAC) was stud-ied. The preparation of an adsorbent with coated chitosan on silica beads Was made. After being cross-linked by dechloride diglycidyl ether, the chitosan coated adsorbent had good chemical stability andhigh mechanic property. When pH> 3. 8, the Cu2+ was adsorbed easily on the chitosan coated adsor-bent with adorbed capacity of 18 mg Cu2+/g chitosan or 0. 91 mg Cu2+ /g beads. The new adsorbentcouId be used for purification of superoxide dismutase. The recovery of SOD was 89% with a purifi-cation factor of 20.