| 大孔树脂纯化鼠曲草总黄酮的研究 |
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| 引用本文： | 石青浩,李荣,姜子涛. 大孔树脂纯化鼠曲草总黄酮的研究[J]. 中国食品添加剂, 2012, 0(3): 93-98 |
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| 作者姓名： | 石青浩 李荣 姜子涛 |
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| 作者单位： | 天津商业大学生物技术与食品科学学院,天津市食品生物技术重点实验室,天津300134 |
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| 基金项目： | 天津市高校科技发展基金项目资助 |
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| 摘 要： | 以鼠曲草黄酮的吸附率、解吸率为指标,考察了六种大孔吸附树脂对鼠曲草中总黄酮的纯化性能,筛选出最佳的大孔吸附树脂,采用动态法分析了吸附流速、pH条件、解吸液乙醇浓度和解吸液流速对吸附解吸的影响,同时采用高效液相色谱法进行分析检测表征了纯化的效果。实验结果表明,大孔吸附树脂AB-8对鼠曲草总黄酮有很好的吸附和解吸性能,并确定了最佳的吸附和解吸条件为:样品液pH=4.0、吸附流速为2BV/h、解吸液乙醇浓度为50%、解吸流速为2BV/h。树脂饱和吸附量为14.7mg/g湿树脂,在此条件下鼠曲草黄酮纯度由原来的28.0%提升到59.4%。
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| 关 键 词： | 鼠曲草 大孔吸附树脂 黄酮 纯化 |
Study on the techniques of purification flavones from Gnaphlium affine by macroporous resins |
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| SHI Qing-hao , LI Rong , JIANG Zi-tao. Study on the techniques of purification flavones from Gnaphlium affine by macroporous resins[J]. China Food Additives, 2012, 0(3): 93-98 |
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| Authors: | SHI Qing-hao LI Rong JIANG Zi-tao |
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| Affiliation: | (Tianjin Key Laboratory of Food Biotechnology,College of Biotechnology and Food Science, Tianjin University of Commerce,Tianjin 300134) |
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| Abstract: | The separation and purification abilities of six kinds of macroporous adsorption resins for flavones in Gnaphlium affine were studied with the adsorption and desorption rates as a profile.The flow of the sample solution,pH,the elution agent(ethanol)concentration,and the washing flow were studied by using dynamic adsorption and desorption.The purification effect was estimated by HPLC.The optimum conditions for purification were:sample pH=4,adsorption flow 2BV/h,elution agent(ethanol)concentration 80%,and washing flow 2BV/h.The saturation adsorption quantity is 14.7mg/g(wet resins).Ethanol of 50% was used as desorption solvent.With these parameters,the purity of product increased from 28.0% to 59.4%. |
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| Keywords: | Gnaphlium affine macroporous resin flavones purification |
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