| 复合肽螯合锌有效螯合率测定方法研究—凝胶过滤色谱络合滴定法 |
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| 引用本文： | 李勇,葛向阳. 复合肽螯合锌有效螯合率测定方法研究—凝胶过滤色谱络合滴定法[J]. 中国食品添加剂, 2012, 0(3): 222-228 |
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| 作者姓名： | 李勇 葛向阳 |
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| 作者单位： | 华中农业大学农业微生物国家重点实验室,武汉,430070 |
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| 基金项目： | 国家农业科技成果转化资金 |
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| 摘 要： | 本文通过模拟动物胃肠道内pH的环境来处理螯合物样品,使螯合物样品中的非络合态以及较小结合力的络合态金属元素以离子状态存在,再通过凝胶过滤法对螯合态和游离态的金属离子进行分离,测定各组分中金属元素的含量,即可计算出样品有效螯合率。具体测定方法为:样品溶解处理时pH调至3.0,离心后的上清液pH调回中性,上柱洗脱时碱性洗脱液为pH 9.0的Clark-lubs缓冲液,酸性洗脱液为pH 2.0HCl溶液。该方法得到的结果变异系数在2%2.5%之间,精密度较好。
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| 关 键 词： | 复合肽螯合锌 凝胶过滤色谱 络合滴定 有效螯合率 |
The chelating rate of peptide chelating zinc and its determination method by gel filtration chromatography complexometric titration |
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| LI Yong , GE Xiang-yang. The chelating rate of peptide chelating zinc and its determination method by gel filtration chromatography complexometric titration[J]. China Food Additives, 2012, 0(3): 222-228 |
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| Authors: | LI Yong GE Xiang-yang |
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| Affiliation: | (State Key Laboratory of Agricultural Microbiology of HuaZhong Agricultural University,Wuhan 430070) |
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| Abstract: | In this paper,samples were chelated in simulating animal gastrointestinal pH environment.The non-chelating compounds as well as the smaller binding metal elements complex were existed in ionic state.Then the chelated complex and free metal ions were separated by gel filtration.The metal element content determined chelation rate.The procedure was:dissolving the samples at pH 3.0 and neutralized pH of the supernatant after centrifugation.The samples were then eluted in Gel column,Clark-lubs buffer with pH 9.0 was selected as alkaline eluent and pH 2.0 HCl solution was used as acidic eluent.The coefficient of variation of the method was between 2% and 2.5%.The method had a better precision. |
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| Keywords: | composite peptide chelated zinc gel filtration chromatography complexometric titration effectual chelation percentage |
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