| 利用玉米芯渣产纤维素酶条件的优化 |
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| 引用本文： | 王成福,李雨,聂永来,李玉珍. 利用玉米芯渣产纤维素酶条件的优化[J]. 中国食品添加剂, 2012, 0(Z1): 129-133 |
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| 作者姓名： | 王成福 李雨 聂永来 李玉珍 |
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| 作者单位： | 山东福田药业有限公司,禹城,251200 |
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| 摘 要： | 以玉米芯渣为培养基料,通过单因素及正交试验,对绿色木霉T9806菌株固态发酵产纤维素酶的培养条件进行摸索。结果表明,最佳培养基组成为:麸皮15.27%,玉米芯渣22.91%,(NH4)2SO40.4%,MgSO40.12%,CaCl20.12%,ZnSO4 0.08%,加水量61.1%。利用最佳培养基配方,在250mL三角瓶中于35℃下恒温培养96h,纤维素酶酶活可达到8132μ/g干曲。
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| 关 键 词： | 玉米芯渣 绿色木霉T9806 固态发酵 纤维素酶 |
Optimality condition on production of cellulose enzyme using corn cob residue |
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| WANG Cheng-fu , LI Yu , NIE Yong-lai , LI Yu-zhen. Optimality condition on production of cellulose enzyme using corn cob residue[J]. China Food Additives, 2012, 0(Z1): 129-133 |
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| Authors: | WANG Cheng-fu LI Yu NIE Yong-lai LI Yu-zhen |
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| Affiliation: | (Shandong Futian Pharmaceutical Co.Ltd.,Yucheng 251200) |
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| Abstract: | Utilizing corn cob residue as substrate the cellulose enzyme solid-state fermentation conditions for Trichoderma viride T9806 was studied through single factor experiments and orthogonal test.The results showed that optimal culture conditions were as follows:medium prepared from 22.91% slag several,15.27% wheat bran,0.4%(NH4)2SO4,0.12%MgSO4,0.12%CaCl2,0.08%ZnSO4 and 61.1% water,at 35℃for 96h.Under these optimal fermentation conditions,cellulose enzyme activity reached 8132u/g dry medium. |
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| Keywords: | corn cob residue trichoderma viride T9806 Solid-state fermentation cellulose enzyme. |
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