多环芳烃分子印迹柱-高效液相色谱荧光检测法快速测定烤肉中15 种多环芳烃

建立一种高效液相色谱-荧光检测（high performance liquid chromatography-fluorescence detection，HPLC-FLD）法同时测定烤肉中15 种多环芳烃（polycyclic aromatic hydrocarbons，PAHs）的方法。样品经超声提取，多环芳烃分子印迹柱（molecular imprinting column of PAHs，MIP-PAHs）净化，通过HPLC分离，外标法定量。结果表明：15 种PAHs在质量浓度1～50 ng/mL范围内与色谱峰面积呈良好的线性关系，r＞0.999 5；在5.0、10.0、25.0 μg/kg 3 个加标水平下，平均回收率为71.1%～98.8%，相对标准偏差为1.0%～5.8%；方法检出限（RS/N=3）为0.33～3.30 μg/kg，定量限（RS/N=10）为1.0～10.0 μg/kg。

Determination of 15 Polycyclic Aromatic Hydrocarbons in Barbecued Meat by Molecularly Imprinted Solid-Phase Extraction Combined with High Performance Liquid Chromatography-Fluorescence Detection

A method for simultaneous determination of 15 polycyclic aromatic hydrocarbons (PAHs) in barbecued meat was developed by high performance liquid chromatography with fluorescence detection (HPLC-FLD). Samples were ultrasonically extracted, and the extract was purified by molecularly imprinted solid-phase extraction (MISPE), separated by HPLC and quantified by external standard method. The results showed that the concentration of 15 PAHs in the range from 1 to 50 ng/mL was linear with the chromatographic peak area with correlation coefficients (r) more than 0.999 5. Average recoveries at spiking levels of 5.0, 10.0 and 25.0 μg/kg ranged from 71.1% to 98.8% with relative standard deviations (RSDs) from 1.0% to 5.8%. The detection limits (RS/N = 3) varied from 0.33 to 3.30 μg/kg and the quantitation limits (RS/N = 10) from 1.0 to 10.0 μg/kg.