Lipase-catalyzed enrichment of long-chain polyunsaturated fatty acids |
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Authors: | Gerald P McNeill Robert G Ackman Stephen R Moore |
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Affiliation: | (1) Unilever Research, Sharnbrook, MK44 1LQ Bedford, UK;(2) Canadian Institute of Fisheries Technology, B3J 2X4 Halifax, Nova Scotia, Canada |
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Abstract: | Lipase hydrolysis was evaluated as a means of selectively enriching long-chain ω3 fatty acids in fish oil. Several lipases
were screened for their ability to enrich total ω-3 acids or selectively enrich either docosahexaenoic acid (DHA) or eicosapentaenoic
acid (EPA). The effect of enzyme concentration, degree of hydrolysis, and fatty acid composition of the feed oil was studied.
Because the materials that were enriched in long-chain ω3 acids were either partial glycerides or free fatty acids, enzymatic
reesterification of these materials to triglycerides by lipase catalysis was also investigated. Hydrolysis of fish oil by
eitherCandida rugosa orGeotrichum candidum lipases resulted in an increase in the content of total ω3 acids from about 30% in the feed oil to 45% in the partial glycerides.
The lipase fromC. rugosa was effective in selectively enriching either DHA or EPA, resulting in a change of either the DHA/EPA ratio or the EPA/DHA
ratio from approximately 1:1 to 5:1. Nonselective reesterification of free fatty acids or partial glycerides that contained
ω3 fatty acids could be achieved at high efficiency (approximately 95% triglycerides in the product) by using immobilizedRhizomucor miehei lipase with continuous removal of water. |
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Keywords: | Candida rugosa DHA enrichment EPA esterification ω 3 fish oil lipase fatty acids Rhizomucor miehei |
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