Nanoliter Liquid Packaging in a Bioresorbable Microsystem by Additive Manufacturing and its Application as a Controlled Drug Delivery Device |
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Authors: | Jongeon Park Arnaud Bertsch Cristina Martin-Olmos Juergen Brugger |
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Affiliation: | 1. Microsystems Laboratory (EPFL-STI-IEM-LMIS1) Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, 1015 Switzerland;2. Center for Advanced Surface Analysis, Institute of Earth Sciences, University of Lausanne (UNIL), Lausanne, 1015 Switzerland
School of Architecture, Civil and Environmental Engineering, EPFL, Lausanne, 1015 Switzerland |
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Abstract: | Precise packaging of nanoliter amounts of liquid in a microsystem is important for many biomedical applications. However, existing liquid encapsulation technologies have limitations in terms of liquid waste, evaporation, trapped bubbles, and liquid degradation. In this study, multiple additive manufacturing techniques for nanoliter liquid packaging in bioresorbable microsystems is used. Two-photon photolithography is used for bioresorbable reservoir fabrication, while inkjet printing (IJP) is used for precise nanoliter liquid packaging. Dual IJP allows for micro-reservoirs to be filled with precise amounts of drug solution and subsequently and rapidly sealed with a layer of lipids mixed with Fe3O4 nanoparticles. Combining these two printing techniques can overcome the previous limitations of liquid encapsulation technologies. To demonstrate the relevance of this technique, a wirelessly activated, bioresorbable multi-reservoir microcapsule that can be used for controlled drug delivery is presented. The microcapsules and their content are shown to be stable during fabrication, storage, and operation. Multiple cargo release events are triggered independently by the local melting of the sealing layer, resulting from magnetically induced Fe3O4 nanoparticle heating. The operation of the capsule is demonstrated in tissue phantoms and in vitro cell cultures. |
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Keywords: | additive manufacturing bioresorbable materials controlled release drug delivery liquid-in-MEMS |
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