载脂蛋白A1和B检测试剂的研制

目的　研制载脂蛋白A1和B检测试剂。方法　采用硫酸葡聚糖沉淀、超速离心、层析等方法。结果　分离的载脂蛋白A1(ApoA1)和B (ApoB) ,经检定证实其为单一成分 ,用它们为抗原 ,免疫绵羊 ,获得ApoA1和ApoB抗血清 ,检定结果与德国Bochinger公司产品一致。用这两种抗血清配制的浊度检测试剂与德国Diasys公司的同类试剂盒进行对照 ,相关良好。结论　所研制的载脂蛋白A1和B检测试剂达到了国外同类产品的质量水平。

Preparation of Kit for Detecting Apoprotein A1 and B

Objective To prepare a kit for detecting apoprotein(Apo) A1 and B. Methods Dextran sulfate precipitation, ultracentrifugation, chromatography, and so on. Results Either Apo A1 and B separated by the above-mentioned methods was proved to be a single component. Anti-Apo Al and anti-Apo B sera were obtained by immunizing sheep with Apo Al and B respectively. The detection results of the 2 kinds of sera were consistent with those of imported products(Bochinger, Germany), and the kit for turbidimetry prepared with them showed good correlation with those manufactured by Diasys Co.(Germany). Conclusion The kit reached the quality level of imported product of the same kind.