| 海洋假单胞菌PT-8胞外硒多糖的制备 |
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| 引用本文： | 李江,叶淑红,王际辉,王晗,肖珊. 海洋假单胞菌PT-8胞外硒多糖的制备[J]. 大连工业大学学报, 2014, 0(4): 250-253 |
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| 作者姓名： | 李江 叶淑红 王际辉 王晗 肖珊 |
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| 作者单位： | 大连工业大学辽宁省食品生物技术重点实验室; |
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| 基金项目： | 国家自然科学基金资助项目(31370554);国家农业科技成果转化资金项目(2013GB2B000090);国家海洋公益性行业科研专项项目(201405003);大连市科技兴海专项资金项目(20140101;20140102) |
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| 摘 要： | 研究了硒的添加量对海洋假单胞菌PT-8硒多糖产量的影响,考察了胞外硒多糖的流变性。对海洋假单胞菌进行发酵培养,确定Na2SeO3加入量为20μg/mL,培养6h后加硒,发酵时间为48h。富硒多糖通过海洋假单胞菌PT-8进行深层富硒发酵,得到富硒多糖产量为7.28g/L,硒质量分数为256.7mg/kg。流变性实验结果表明,在不同浓度、温度和剪切力条件下,多糖的黏度高于硒多糖黏度。多糖在pH为4时黏度达到最大,为96mPa·s;硒多糖在pH为5时黏度达到最大,为80mPa·s。初步确定该多糖为酸性多糖。
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| 关 键 词： | 假单胞菌PT-8 胞外硒多糖 流变性 |
Production of selenium-enriched exopolysaccharide by marine Pseudomonas PT-8 |
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| LI Jiang;YE Shuhong;WANG Jihui;WANG Han;XIAO Shan. Production of selenium-enriched exopolysaccharide by marine Pseudomonas PT-8[J]. Journal of Dalian Dalian Polytechnic University, 2014, 0(4): 250-253 |
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| Authors: | LI Jiang YE Shuhong WANG Jihui WANG Han XIAO Shan |
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| Affiliation: | LI Jiang;YE Shuhong;WANG Jihui;WANG Han;XIAO Shan;Liaoning Provincial Key Laboratory of Food Biotechnology,Dalian Polytechnic University; |
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| Abstract: | The influence of selenium concentration on the enriched-selenium exopolysaccharides(Se-EPS)production by Pseudomonas PT-8 was studied. The yield of enriched-selenium exopolysaccharide was 7.28g/L,Se-enriched amount was 256.7mg/kg when Se-concentration was20μg/mL,Se-added time 6th h,shake culture 48h.EPS showed stronger rheological property than Se-EPS.The viscosity of EPS at pH 4was 96mPa·s,but the viscosity of Se-EPS was 80mPa·s.It is primarily confirmed that Se-EPS and EPS should be acidic polysaccharide. |
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| Keywords: | Pseudomonas PT-8 enriched-selenium exopolysaccharides rheological property |
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