| 酶反应产物中人参稀有皂苷F2与C-Mc的分离 |
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| 引用本文： | 李永淳,刘春莹,鱼红闪,金凤燮.酶反应产物中人参稀有皂苷F2与C-Mc的分离[J].大连工业大学学报,2014(6):420-422. |
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| 作者姓名： | 李永淳 刘春莹 鱼红闪 金凤燮 |
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| 作者单位： | 大连工业大学生物工程学院; |
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| 基金项目： | “重大新药创新”科技重大专项(2012ZX09503001-003) |
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| 摘 要： | 原人参二醇类皂苷混合物经人参皂苷酶生物转化后可生成F2、C-Mc等7~10种稀有人参皂苷。天然人参中不含人参皂苷C-Mc,且F2的含量极低。在生物转化所得的人参二醇类皂苷酶反应产物中,分离纯化得到稀有人参皂苷F2与C-Mc,并进行HPLC检测。反应后得到粗产品40g,经脱糖脱色和硅胶柱分离后,成功得到稀有人参皂苷F23.49g,纯度为98.2%,得率8.72%;得到C-Mc 0.70g,纯度为82.2%,得率为1.80%。成功分离出了F2和C-Mc制品,建立了初步的分离制备方法。
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| 关 键 词： | 稀有人参皂苷 生物转化 硅胶柱层析 HPLC |
Separation of ginsenoside F_2 and C-Mc from the product of ginsenoside biotransformation |
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| LI Yongchun;LIU Chunying;YU Hongshan;JIN Fengxie.Separation of ginsenoside F_2 and C-Mc from the product of ginsenoside biotransformation[J].Journal of Dalian Dalian Polytechnic University,2014(6):420-422. |
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| Authors: | LI Yongchun;LIU Chunying;YU Hongshan;JIN Fengxie |
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| Affiliation: | LI Yongchun;LIU Chunying;YU Hongshan;JIN Fengxie;School of Biological Engineering,Dalian Polytechnic University; |
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| Abstract: | Protopanaxadiol ginsenosides were transformed into 7-10 types of rare ginsenosides such as F2 and C-Mc by the ginsenoside enzyme.F2 has a very low content in the natural and C-Mc does not exist in natural ginseng.The crude product transformed from protopanaxadiol ginsenosides(PPD)was separated and purified.Silica gel chromatography was applied to separate and purify 40 g crude product,which has been desugared and decolored.3.49 g ginsenoside F2 with the purity of 98.2%was obtained,and its yield was 8.72%.0.70 g C-Mc with the purity of 82.2% was also obtained,and its yield was 1.80%.This experiment has successfully established a preliminary method of preparing and separating on F2 and C-Mc. |
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| Keywords: | rare ginsenoside biotransformation silica gel chromatography HPLC |
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