金铁锁毛状根悬浮培养动力学

通过测定金铁锁毛状根在不同培养阶段的生物量、皂苷含量以及培养基中碳源、氮源、磷源的消耗,研究悬浮培养毛状根的生长、营养消耗与次级代谢产物积累之间的规律。结果表明,金铁锁毛状根悬浮培养周期约为32d,生物量和皂苷质量分数分别达到12.33g/L(DW)和0.97%;毛状根生长与皂苷积累关系为半生长偶联型,分别在22和30d达到峰值0.36g/(L·d)和1.00mg/(L·d)。在培养的第22天,培养基中碳源和磷源的质量浓度最低,毛状根的生物量则达到最大值;氮源在毛状根生长处于静止期时(0~4d)迅速消耗,至20d(对数生长期)时消耗完毕。结果提示,通过补料方式可以延长毛状根悬浮培养的生长周期。

Kinetics in suspension culture of Psammosilene tunicoides hairy roots

To explain the relationship among the growth,nutrients and secondary metabolites accumulation,the kinetics of the suspension culture of P.tunicoides hairy roots were investigated including cell growth,accumulation of saponins and consumption of the carbon,nitrogen and phosphorus in the medium.The hairy roots suspension culture period was about 32 dby which the maximum biomass(DW)and the saponin content reached to 12.33g/L and 0.97%,respectively.The accumulation of saponines was in a semi-coupling mode with hairy roots growth,and they reached their peak of 0.36g/(L·d)and 1.00mg/(L·d)on 22 and 30dof culture,respectively.By 22 dof culture,the contents of both carbon and phosphorus in the media declined to the lowest,however,a maximum biomass of hairy roots was harvested at the same time.Moreover,a rapid consumption of nitrogen source was found at the static stage of hairy roots growth(0-4d),and its content declined to blank by 22 dof culture(period of exponential growth).The results suggested that the extending of P.tunicoides hairy roots growth period would be realized by feeding of necessary nutrients during culture.