| 水质净化芽孢杆菌的筛选及培养条件优化 |
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| 引用本文： | 李加双,刘彤,黄忠刚,肖珊,叶淑红,王晗,王际辉.水质净化芽孢杆菌的筛选及培养条件优化[J].大连工业大学学报,2014(6):404-408. |
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| 作者姓名： | 李加双 刘彤 黄忠刚 肖珊 叶淑红 王晗 王际辉 |
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| 作者单位： | 大连工业大学食品学院;大连市水产技术推广总站;大连博仕奥生物科技有限公司; |
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| 基金项目： | 国家农业科技成果转化资金项目(2013GB2B000090);辽宁特聘教授资助项目(2011[191]);国家海洋公益性行业科研专项项目(201405003);大连市科技兴海专项资金项目(20140101;20140102) |
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| 摘 要： | 从养殖池底的海泥筛选出两株具有净化水质的细菌T01、T02,对其进行生理生化鉴定,确定均为芽孢杆菌。对T01、T02及复合菌株(体积比1∶1)降解氨氮和亚硝酸盐氮能力进行测试,菌株T01、T02均具有降解氨氮和亚硝酸盐氮的能力。选择生长状况较好的T02进行培养条件优化,在葡萄糖10g/L、淀粉15g/L、蛋白胨5.0g/L、豆粕10g/L、磷酸氢二钾1.5g/L、磷酸二氢钾1.5g/L、硫酸镁1.0g/L、碳酸钙1.0g/L的培养基条件下活菌数达到最高,为1.54×109 cfu/mL。在接种量1%、培养温度30℃、转速180r/min、装液量2/5时,活菌数达到最高,为1.63×109 cfu/mL。
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| 关 键 词： | 芽孢杆菌 水质净化 培养条件 |
Selection of Bacillusfor water purification and culture condition optimization |
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| LI Jiashuang;LIU Tong;HUANG Zhonggang;XIAO Shan;YE Shuhong;WANG Han;WANG Jihui.Selection of Bacillusfor water purification and culture condition optimization[J].Journal of Dalian Dalian Polytechnic University,2014(6):404-408. |
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| Authors: | LI Jiashuang;LIU Tong;HUANG Zhonggang;XIAO Shan;YE Shuhong;WANG Han;WANG Jihui |
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| Affiliation: | LI Jiashuang;LIU Tong;HUANG Zhonggang;XIAO Shan;YE Shuhong;WANG Han;WANG Jihui;School of Food Science and Technology,Dalian Polytechnic University;Dalian Fisheries Technology Extension Station;Dalian Boss-all Biological Science and Technology Limited Company; |
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| Abstract: | Strains T01 and T02 with water purifying function were isolated from sea-mud in culture pond bottom and identified as Bacillus.Both strains were capable of degrading ammonia nitrogen and nitrite nitrogen.The number of living bacteria achieved the highest at 1.54×109 cfu/mL when the glucose was 10g/L,starch 15g/L,peptone 5g/L,soybean meal 10g/L,potassium hydrogen phosphate 1.5g/L,potassium dihydrogen phosphate 1.5g/L,magnesium sulfate 1g/L,calcium carbonate 1g/L.The optimum growth occurred at 1%inoculation amount in 2/5fluid volume and at30 ℃,180r/min,in which the highest cells was 1.63×109 cfu/mL. |
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| Keywords: | Bacillus water purification culture condition |
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