生化分离过程的新探索──双水相分配与相关技术的集成化

探讨了双水相分配与相关技术 (如电泳、层析、温度诱导相分离、亲和技术、磁场作用、超声波作用等 )的集成化过程 ,阐述了生化分离过程中集成化的趋势和优势 .不同的生化分离技术通过有效的组合 ,实现集成化 ,促进了相互间的交叉、渗透和融合 ,解决了单一技术本身存在的难点问题 ,有效地提高了分离效率 ,并对形成新分离过程提供了思路 .

NOVEL TECHNOLOGY IN BIOSEPARATION PROCESS--INTEGRATION OF AQUEOUS TWO-PHASE PARTITIONING WITH RELATED TECHNIQUES

The successful commercialization of biotechnology depends strongly on the development of separation processes. A novel technology to combine different separation operations into a single stage to give higher yield, greater selectivity and optimum scheme is described as the integration of bioseparation processes. Based on the integration of aqueous two phase partitioning with related techniques, the trends and advantages of integration in bioseparation processes are investigated. Some disadvantages in separation process are resolved after combining aqueous two phase partitioning with temperature induced phase separation,magnetic field,ultrasonic and colloidal gas aphron technology. Integration of aqueous two phase partitioning with affinity precipitation and chromatography increases significantly the efficiency of separation and simplifies the separation process. Furthermore,a new idea is developed to innovate bioseparation technology when aqueous two phase partitioning is integrated into bioconversation,chemical permeabilization or electrophoresis. In general, integration of bioseparation processes provides a good way of combining different techniques to improve and optimize the whole separation process.