| 茎瘤芥叶叶绿素提取工艺研究 |
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| 引用本文： | 李敏,余辉,姚成强. 茎瘤芥叶叶绿素提取工艺研究[J]. 中国调味品, 2012, 37(2): 79-82 |
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| 作者姓名： | 李敏 余辉 姚成强 |
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| 作者单位： | 长江师范学院生命科学与技术学院,重庆涪陵,408000 |
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| 基金项目： | 重庆市教委科技项目:利用茎瘤芥(榨菜)叶和皮筋提取叶绿素的技术研究[KJ101316] |
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| 摘 要： | 以茎瘤芥叶为原料,采用冷冻干燥技术对叶片进行预处理后,采用单因素试验和正交试验研究不同的浸提剂、乙醇浓度、料液比、浸提温度和浸提时间对茎瘤芥叶叶绿素提取率的影响,并优化茎瘤芥叶叶绿素提取工艺.结果表明:有机溶剂提取茎瘤芥叶叶绿素优化工艺为料液比1∶50,提取溶剂为乙醇体积分数70％的乙醇/丙酮溶液,50℃水浴温度浸提3h,叶绿素提取率为1.08％.
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| 关 键 词： | 茎瘸芥叶 有机溶剂 叶绿素 提取工艺 |
Study on extracting technology of chlorophyll from leaves of Brassica juncea var.tumida Tsen et Lee |
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| LI Min , YU Hui , YAO Cheng-qiang. Study on extracting technology of chlorophyll from leaves of Brassica juncea var.tumida Tsen et Lee[J]. China Condiment, 2012, 37(2): 79-82 |
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| Authors: | LI Min YU Hui YAO Cheng-qiang |
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| Affiliation: | (Life Science and Technology College of Yangtze Normal University,Fuling 408000,China) |
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| Abstract: | In this study,freeze drying technique was applied to pretreat the leaves of Brassica juncea var.tumida Tsen et Lee.The chlorophyll’s extracting ratio was effected by the different extractant,alcohol concentration,solid-liquid ratio,extracting temperature,extracting time.These studies’ methods were single factor test and orthogonal test.Meanwhile the extracting technology was optimized.The optimized extracting conditions were as follows: solid-liquid ratio of 1∶50;alcohol concentration of 70%(vol) alcohol /acetone solvent,50 ℃ waterbath extracting time of 3 h.The chlorophyll’s extracting ratio was 1.08%. |
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| Keywords: | leaves of Brassica juncea var.tumida Tsen et Lee organic solvent chlorophyll extration techology |
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