同步硝化反硝化细菌的鉴定与脱氮特性研究

依据同步硝化反硝化脱氮原理,以生活污水处理厂污泥为种泥,利用15℃低温摇床富集并分离出同步硝化反硝化细菌（SNDB-5）,采用传统与现代分子生物学相结合的手段对其鉴定,以确定其分类地位;利用水浴摇床研究SNDB-5对硝化细菌液体培养基NO2--N的去除效果。结果表明：SNDB-5与Pseudomonas mendocina同源性达99.0%,形态特征、革兰氏染色及生理生化与Pseudomonas mendocina最相似,属假单胞菌属;摇床培养反应条件显示,当NO2--N浓度加大到150mg/L时,菌株SNDB-5的活性受到了抑制。

Identification and Characteristics Analysis of Simultaneous Nitrifying-Denitrifying Bacteria and Nitrogen Removing

Based on the principle of simultaneously nitrifying-denitrifying nitrogen removing,sewage treatment plant wastewater sludge was used as inoculum,and the continuous low temperature shaker was used to enrich the simultaneously nitrifying-denitrifying nitrogen removing bacteria（SNDB-5） at 15℃.Traditional and modern molecular biology methods were adopted to determine the taxonomic status of the isolated bacteria which was named SNDB-5.Water bath shaker was used to analyze the NO2——N removal in the nitrifying bacteria culture medium.The results show that SNDB-5 has 99.0 % homology with Pseudomonas mendocina.Morphological,gram staining and biochemical characteristics are also similar to Pseudomonas mendocina.Incubating reaction conditions in shaker shows that SNDB-5 activity is inhibited when the concentration of NO2——N increases to 150 mg/L.