| 人精浆前列腺特异抗原的分离纯化及鉴定 |
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| 引用本文： | 黄学忠,武建国,虞伟. 人精浆前列腺特异抗原的分离纯化及鉴定[J]. 中国生物制品学杂志, 1996, 0(4) |
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| 作者姓名： | 黄学忠 武建国 虞伟 |
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| 作者单位： | 解放军第118医院!温州325000(黄学忠)，全军医学检验中心!南京210002(武建国，虞伟) |
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| 摘 要： | 用PEG－SephadexG－150两步法，从精浆中分离纯化前列腺特异抗原（PSA）。精浆标本先经PEG粗提，约去除80％的杂蛋白，再经柱层析，进一步提纯，提纯产物经SDS－PAGE及ELISA法证实为免疫纯。
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| 关 键 词： | 前列腺特异抗原 精浆 |
Separation, Purification and Characterization of Prostate Specific Antigen from Human Seminal Plasma |
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| Huang Xuezhong et al. Separation, Purification and Characterization of Prostate Specific Antigen from Human Seminal Plasma[J]. Chinese Journal of Bilogicals, 1996, 0(4) |
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| Authors: | Huang Xuezhong et al |
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| Abstract: | Prostate specific antigen (PSA) was separated and purified from human seminal plasma by PEG precipitation and Sephadex G - 150 chromatography. After about 80% of non-specific protein was removed by PEG precipitation, PSA was further purified by column chromatography. SDS - PAGE and ELISA proved that the final PSA was immunologically pure. |
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| Keywords: | Prostate specific antigen (PSA) Seminal plasma |
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