首页 | 本学科首页   官方微博 | 高级检索  
     


High-Throughput Gene and Protein Analysis Revealed the Response of Disc Cells to Vitamin D,Depending on the VDR FokI Variants
Authors:Alessandra Colombini  Paola De Luca  Davide Cangelosi  Carlotta Perucca Orfei  Enrico Ragni  Marco Vigan  Michela Malacarne  Mauro Castagnetta  Marco Brayda-Bruno  Domenico Coviello  Laura de Girolamo
Affiliation:1.Orthopaedic Biotechnology Lab, IRCCS Istituto Ortopedico Galeazzi, 20161 Milan, Italy; (P.D.L.); (C.P.O.); (E.R.); (M.V.); (L.d.G.);2.Laboratorio di Genetica Umana, IRCCS Istituto Giannina Gaslini, 16147 Genoa, Italy; (D.C.); (M.M.); (D.C.);3.Laboratorio di Istocompatibilità/IBMDR, Ospedali Galliera, 16128 Genoa, Italy;4.Scoliosis Unit, Department of Orthopedics and Traumatology-Spine Surgery III, IRCCS Istituto Ortopedico Galeazzi, 20161 Milan, Italy;
Abstract:Vitamin D showed a protective effect on intervertebral disc degeneration (IDD) although conflicting evidence is reported. An explanation could be due to the presence of the FokI functional variant in the vitamin D receptor (VDR), observed as associated with spine pathologies. The present study was aimed at investigating—through high-throughput gene and protein analysis—the response of human disc cells to vitamin D, depending on the VDR FokI variants. The presence of FokI VDR polymorphism was determined in disc cells from patients with discopathy. 1,25(OH)2D3 was administered to the cells with or without interleukin 1 beta (IL-1β). Microarray, protein arrays, and multiplex protein analysis were performed. In both FokI genotypes (FF and Ff), vitamin D upregulated metabolic genes of collagen. In FF cells, the hormone promoted the matrix proteins synthesis and a downregulation of enzymes involved in matrix catabolism, whereas Ff cells behaved oppositely. In FF cells, inflammation seems to hamper the synthetic activity mediated by vitamin D. Angiogenic markers were upregulated in FF cells, along with hypertrophic markers, some of them upregulated also in Ff cells after vitamin D treatment. Higher inflammatory protein modulation after vitamin D treatment was observed in inflammatory condition. These findings would help to clarify the clinical potential of vitamin D supplementation in patients affected by IDD.
Keywords:intervertebral disc  vitamin D receptor gene polymorphism  vitamin D  inflammation  gene profile  protein profile
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号