| 蜡状芽抱杆菌壬基苯酚降解酶的分离纯化及其酶学性质(英文) |
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| 引用本文： | 杨革,张营,白艳芬. 蜡状芽抱杆菌壬基苯酚降解酶的分离纯化及其酶学性质(英文)[J]. 中国化学工程学报, 2011, 19(4): 644-648. DOI: 10.1016/S1004-9541(11)60035-9 |
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| 作者姓名： | 杨革 张营 白艳芬 |
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| 作者单位： | 1. College of Textiles Tianjin Polytechnic University, Tianjin 300160, China;2. Key Lab of Biogeology and Environmental Geology of Ministry of Education, China University of Geosciences, Wuhan 430074, China |
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| 基金项目： | Supported by the Fund of Open Subject of Key Lab of Biogeology and Environmental Geology of Ministry of Education(BGEG1006) |
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| 摘 要： | An extracellular NP-degrading enzyme secreted by Bacillus cereus.Frankland was purified to homogeneity by a combination of ammonium sulfate precipitation,Phenyl-Sepharose hydrophobic-interaction chromatography and DEAE anion-exchange chromatography.On SDS(sodium dodecyl sulfate)-polyacrylamide gel electrophoresis analysis,the purified enzyme showed a relative molecular mass of 58.3 kDa.The depolymerzation of subunits was accompanied with the loss of NP-degrading enzyme activity,and removing denaturing factors by dialysis could restore the dimer structure and enzymatic activity.The enzyme had an isoelectric point of 5.5 and an optimal temperature of 60℃,and was the most active at pH 6.0.The enzymatic activity was stable at pH 4-8 and inhibited by Cu2+.TenN-terminal amino acids were determined to be ASVNSIKIGY,demonstrating that the purified enzyme was a novel one.The hydrolysis pattern of the purified enzyme indicated that the NP-degrading enzyme was an endo NP-degrading enzyme.The extraordinary thermo-stability provided the enzyme with a good prospect to be used as a new tool for clean-production process for textile industry.
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| 关 键 词： | 层析纯化 降解酶 NP 芽孢杆菌 壬基酚 十二烷基硫酸钠 表征 蜡样芽胞杆菌 |
| 收稿时间： | 2010-04-07 |
| 修稿时间： | 2011-4-18 |
Purification and Characterization of a Nonylphenol (NP)-degrading Enzyme from Bacillus cereus. Frankland |
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| Ge YANG,Ying ZHANG,Yanfen BAI. Purification and Characterization of a Nonylphenol (NP)-degrading Enzyme from Bacillus cereus. Frankland[J]. Chinese Journal of Chemical Engineering, 2011, 19(4): 644-648. DOI: 10.1016/S1004-9541(11)60035-9 |
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| Authors: | Ge YANG Ying ZHANG Yanfen BAI |
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| Affiliation: | 1. College of Textiles Tianjin Polytechnic University, Tianjin 300160, China;2. Key Lab of Biogeology and Environmental Geology of Ministry of Education, China University of Geosciences, Wuhan 430074, China |
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| Abstract: | An extracellular NP-degrading enzyme secreted by Bacillus cereus. Frankland was purified to homogeneity by a combination of ammonium sulfate precipitation, Phenyl-Sepharose hydrophobic-interaction chromatography and DEAE anion-exchange chromatography. On SDS (sodium dodecyl sulfate)-polyacrylamide gel electrophoresis analysis, the purified enzyme showed a rela-tive molecular mass of 58.3 kDa. The depolymerzation of subunits was accompanied with the loss of NP-degrading enzyme activity, and removing denaturing factors by dialysis could restore the dimer structure and enzymatic activity. The enzyme had an isoelectric point of 5.5 and an optimal temperature of 60 °C, and was the most active at pH 6.0. The enzymatic activity was stable at pH 4-8 and inhibited by Cu2+. Ten N-terminal amino acids were determined to be ASVNSIKIGY, demonstrating that the purified enzyme was a novel one. The hydrolysis pattern of the purified enzyme indicated that the NP-degrading enzyme was an endo NP-degrading enzyme. The extraordinary thermo-stability provided the enzyme with a good prospect to be used as a new tool for clean-production process for textile industry. |
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| Keywords: | nonylphenol (NP) Bacillus cereus Frankland NP-degrading enzyme purification characterization |
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