钙和磷对小麦胚芽愈伤组织维生素E积累的影响

考查培养基中钙元素和磷元素的含量对小麦胚芽愈伤组织维生素E积累的影响,建立维生素E积累和细胞生长的动力学模型.以次生代谢产物维生素E积累最多为目标,通过实验优化确定钙磷含量的最佳组合.结果表明,当B5培养基中CaCl_2·2H_2O为150mg/L,NaH_2PO_4·H_2O为300mg/L时,有利于维生素E积累.维生素E积累的动力学模型的拟合度为97.96%,细胞干重的动力学模型拟合度为95.57%,非线性关系良好.大量元素钙和磷对小麦胚芽愈伤组织维生素E积累有影响,磷元素较钙元素的影响显著.

Effect of calcium and phosphorus on accumulation of vitamin E in wheat embryo callus

The effect of the content of calcium element and phosphorus element in the culture on accumulated vitamin E of wheat embryo callus was studied.The mathematical models were established to describe growth kinetics and accumulation of vitamin E for wheat embryo callus cells.Based on the most accumulation of secondary metabolites Vitamin E as an object,the best combination of Ca element, P element in medium was selected by test optimum.The results showed that the production of vitamin E in B, medium was most at the CaCl_2·2H_2O 150mg/L and NaH_2PO_4 · H_2O 300mg/L.The fitting degree of kinetic model of Vitamin E accumulation was 97.96％,and the fitting degree of kinetic model of cell growth was 95.57％, nonlinear relation was well.The trace element Ca and P affect vitamin E accumulates in medium for wheat germ callus,the influence of element P was more remarkable than that of element Ca.