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啤酒酵母蛋白酶解物分子量分布研究初探
引用本文:郭卫芸, 杜冰, 李燕杰, 杨公明. 啤酒酵母蛋白酶解物分子量分布研究初探[J]. 食品工业科技, 2009, (12): 110-112.
作者姓名:郭卫芸  杜冰  李燕杰  杨公明
摘    要:凝胶层析分离经胰蛋白酶酶解啤酒酵母后得到的多肽粗粉,并采用SDS-PAGE电泳测定啤酒酵母多肽分子量,发现其中含有16~26、5、1·4kDa等三种不同分子量水平的蛋白组分,酶解得率分别为27·08%、11·55%、17·99%。 

关 键 词:啤酒酵母蛋白  胰蛋白酶酶解物  凝胶层析  SDS-PAGE电泳  分子量分布

Primary research of molecular weight distribution of protein hydrolysate in brewer yeast
GUO Wei-yun,DU ging,LI Yan-jie,YANG Gong-ming. Primary research of molecular weight distribution of protein hydrolysate in brewer yeast[J]. Science and Technology of Food Industry, 2009, 0(12): 110-112
Authors:GUO Wei-yun  DU ging  LI Yan-jie  YANG Gong-ming
Abstract:Gel chromatography was used to separate the polypeptide powder which was hydrolysated by trypsin in brewer yeast, and SDS-PAGE electrophoresis was adopted to determine the molecular weight of the polypeptide. The molecular weight of these three components was respectively 16-26,5,1.4kDa, and their gain rate by trypsin hydrolysate were respectively 27.08% ,11.55% and 17.99%.
Keywords:protein of brewer yeast  trypsin hydrolysate  gel chromatography  SDS- PAGE electrophoresis  molecular weight distribution
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